Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Human induced pluripotent stem cells free of vector and transgene sequences.

Science (New York, N.Y.) | May 8, 2009

http://www.ncbi.nlm.nih.gov/pubmed/19325077

Reprogramming differentiated human cells to induced pluripotent stem (iPS) cells has applications in basic biology, drug development, and transplantation. Human iPS cell derivation previously required vectors that integrate into the genome, which can create mutations and limit the utility of the cells in both research and clinical applications. We describe the derivation of human iPS cells with the use of nonintegrating episomal vectors. After removal of the episome, iPS cells completely free of vector and transgene sequences are derived that are similar to human embryonic stem (ES) cells in proliferative and developmental potential. These results demonstrate that reprogramming human somatic cells does not require genomic integration or the continued presence of exogenous reprogramming factors and removes one obstacle to the clinical application of human iPS cells.

Pubmed ID: 19325077 RIS Download

Mesh terms: Cell Differentiation | Cell Shape | Cellular Reprogramming | Clone Cells | Embryonic Stem Cells | Epstein-Barr Virus Nuclear Antigens | Fibroblasts | Gene Expression Profiling | Genetic Vectors | Humans | Plasmids | Pluripotent Stem Cells | Transcription Factors | Transfection | Transgenes

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: P01 GM081629
  • Agency: NIGMS NIH HHS, Id: P01 GM081629
  • Agency: NIGMS NIH HHS, Id: P01 GM081629-01A1
  • Agency: NCRR NIH HHS, Id: P51 RR000167

Addgene (Reagent, Plasmid)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.