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Nutrient-dependent mTORC1 association with the ULK1-Atg13-FIP200 complex required for autophagy.

Autophagy is an intracellular degradation system, by which cytoplasmic contents are degraded in lysosomes. Autophagy is dynamically induced by nutrient depletion to provide necessary amino acids within cells, thus helping them adapt to starvation. Although it has been suggested that mTOR is a major negative regulator of autophagy, how it controls autophagy has not yet been determined. Here, we report a novel mammalian autophagy factor, Atg13, which forms a stable approximately 3-MDa protein complex with ULK1 and FIP200. Atg13 localizes on the autophagic isolation membrane and is essential for autophagosome formation. In contrast to yeast counterparts, formation of the ULK1-Atg13-FIP200 complex is not altered by nutrient conditions. Importantly, mTORC1 is incorporated into the ULK1-Atg13-FIP200 complex through ULK1 in a nutrient-dependent manner and mTOR phosphorylates ULK1 and Atg13. ULK1 is dephosphorylated by rapamycin treatment or starvation. These data suggest that mTORC1 suppresses autophagy through direct regulation of the approximately 3-MDa ULK1-Atg13-FIP200 complex.

Pubmed ID: 19211835

Authors

  • Hosokawa N
  • Hara T
  • Kaizuka T
  • Kishi C
  • Takamura A
  • Miura Y
  • Iemura S
  • Natsume T
  • Takehana K
  • Yamada N
  • Guan JL
  • Oshiro N
  • Mizushima N

Journal

Molecular biology of the cell

Publication Data

April 1, 2009

Associated Grants

None

Mesh Terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acids
  • Animals
  • Autophagy
  • Cell Line
  • Cell Membrane
  • Humans
  • Intracellular Signaling Peptides and Proteins
  • Mice
  • Molecular Weight
  • Multiprotein Complexes
  • Phosphorylation
  • Protein Binding
  • Protein Kinases
  • Protein Transport
  • Protein-Serine-Threonine Kinases
  • Proteins
  • TOR Serine-Threonine Kinases
  • Transcription Factors