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Yeast Mph1 helicase dissociates Rad51-made D-loops: implications for crossover control in mitotic recombination.

Genes & development | Jan 1, 2009

http://www.ncbi.nlm.nih.gov/pubmed/19136626

Eukaryotes possess mechanisms to limit crossing over during homologous recombination, thus avoiding possible chromosomal rearrangements. We show here that budding yeast Mph1, an ortholog of human FancM helicase, utilizes its helicase activity to suppress spontaneous unequal sister chromatid exchanges and DNA double-strand break-induced chromosome crossovers. Since the efficiency and kinetics of break repair are unaffected, Mph1 appears to channel repair intermediates into a noncrossover pathway. Importantly, Mph1 works independently of two other helicases-Srs2 and Sgs1-that also attenuate crossing over. By chromatin immunoprecipitation, we find targeting of Mph1 to double-strand breaks in cells. Purified Mph1 binds D-loop structures and is particularly adept at unwinding these structures. Importantly, Mph1, but not a helicase-defective variant, dissociates Rad51-made D-loops. Overall, the results from our analyses suggest a new role of Mph1 in promoting the noncrossover repair of DNA double-strand breaks.

Pubmed ID: 19136626 RIS Download

Mesh terms: Alleles | Crossing Over, Genetic | DEAD-box RNA Helicases | DNA Breaks, Double-Stranded | DNA Repair | DNA, Fungal | Mitosis | Nucleic Acid Conformation | Rad51 Recombinase | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Sister Chromatid Exchange

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Associated grants

  • Agency: NIEHS NIH HHS, Id: ES07061
  • Agency: NIGMS NIH HHS, Id: GM20056
  • Agency: NIGMS NIH HHS, Id: GM53738
  • Agency: NIGMS NIH HHS, Id: GM57814
  • Agency: NIGMS NIH HHS, Id: GM80600
  • Agency: PHS HHS, Id: GR076476
  • Agency: NIEHS NIH HHS, Id: R01 ES007061
  • Agency: NIGMS NIH HHS, Id: R01 GM020056
  • Agency: NIGMS NIH HHS, Id: R01 GM020056-32
  • Agency: NIGMS NIH HHS, Id: R01 GM020056-33
  • Agency: NIGMS NIH HHS, Id: R01 GM020056-34
  • Agency: NIGMS NIH HHS, Id: R01 GM053738
  • Agency: NIGMS NIH HHS, Id: R01 GM076020
  • Agency: NIGMS NIH HHS, Id: R01 GM076020-04
  • Agency: NIGMS NIH HHS, Id: R01 GM080600
  • Agency: NIGMS NIH HHS, Id: R01 GM080600-03

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