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Systematic and integrative analysis of large gene lists using DAVID bioinformatics resources.

DAVID bioinformatics resources consists of an integrated biological knowledgebase and analytic tools aimed at systematically extracting biological meaning from large gene/protein lists. This protocol explains how to use DAVID, a high-throughput and integrated data-mining environment, to analyze gene lists derived from high-throughput genomic experiments. The procedure first requires uploading a gene list containing any number of common gene identifiers followed by analysis using one or more text and pathway-mining tools such as gene functional classification, functional annotation chart or clustering and functional annotation table. By following this protocol, investigators are able to gain an in-depth understanding of the biological themes in lists of genes that are enriched in genome-scale studies.

Pubmed ID: 19131956


  • Huang da W
  • Sherman BT
  • Lempicki RA


Nature protocols

Publication Data

January 9, 2009

Associated Grants

  • Agency: NCI NIH HHS, Id: N01-CO-56000

Mesh Terms

  • Computational Biology
  • Data Interpretation, Statistical
  • Genes
  • Genomics
  • Internet
  • Software