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Three distinct condensin complexes control C. elegans chromosome dynamics.

BACKGROUND: Condensin complexes organize chromosome structure and facilitate chromosome segregation. Higher eukaryotes have two complexes, condensin I and condensin II, each essential for chromosome segregation. The nematode Caenorhabditis elegans was considered an exception, because it has a mitotic condensin II complex but appeared to lack mitotic condensin I. Instead, its condensin I-like complex (here called condensin I(DC)) dampens gene expression along hermaphrodite X chromosomes during dosage compensation. RESULTS: Here we report the discovery of a third condensin complex, condensin I, in C. elegans. We identify new condensin subunits and show that each complex has a conserved five-subunit composition. Condensin I differs from condensin I(DC) by only a single subunit. Yet condensin I binds to autosomes and X chromosomes in both sexes to promote chromosome segregation, whereas condensin I(DC) binds specifically to X chromosomes in hermaphrodites to regulate transcript levels. Both condensin I and II promote chromosome segregation, but associate with different chromosomal regions during mitosis and meiosis. Unexpectedly, condensin I also localizes to regions of cohesion between meiotic chromosomes before their segregation. CONCLUSIONS: We demonstrate that condensin subunits in C. elegans form three complexes, one that functions in dosage compensation and two that function in mitosis and meiosis. These results highlight how the duplication and divergence of condensin subunits during evolution may facilitate their adaptation to specialized chromosomal roles and illustrate the versatility of condensins to function in both gene regulation and chromosome segregation.

Pubmed ID: 19119011

Authors

  • Csankovszki G
  • Collette K
  • Spahl K
  • Carey J
  • Snyder M
  • Petty E
  • Patel U
  • Tabuchi T
  • Liu H
  • McLeod I
  • Thompson J
  • Sarkeshik A
  • Sarkesik A
  • Yates J
  • Meyer BJ
  • Hagstrom K

Journal

Current biology : CB

Publication Data

January 13, 2009

Associated Grants

  • Agency: NCRR NIH HHS, Id: P41 RR011823
  • Agency: NIGMS NIH HHS, Id: R01 GM030702
  • Agency: NIGMS NIH HHS, Id: R01 GM030702-28
  • Agency: NIGMS NIH HHS, Id: R01 GM076378
  • Agency: NIGMS NIH HHS, Id: R01 GM079533
  • Agency: NIGMS NIH HHS, Id: R01 GM30702
  • Agency: NIGMS NIH HHS, Id: T32 GM07544
  • Agency: Howard Hughes Medical Institute, Id:
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Adenosine Triphosphatases
  • Animals
  • Caenorhabditis elegans
  • Cell Nucleus Division
  • Chromosome Segregation
  • Chromosomes
  • DNA-Binding Proteins
  • Dosage Compensation, Genetic
  • Evolution, Molecular
  • Gene Expression Regulation
  • Immunoprecipitation
  • Models, Biological
  • Multiprotein Complexes
  • Proteomics
  • RNA Interference