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BRCA1-associated protein 1 interferes with BRCA1/BARD1 RING heterodimer activity.

The breast and ovarian tumor suppressor BRCA1 constitutes a RING heterodimer E3 ligase with BARD1. BRCA1-associated protein 1 (BAP1) is a ubiquitin COOH-terminal hydrolase that was initially identified as a protein that bound to the RING finger domain of BRCA1. However, how BAP1 contributes to the E3 activity of BRCA1/BARD1 is unclear. Here, we report that BAP1 interacts with BARD1 to inhibit the E3 ligase activity of BRCA1/BARD1. Domains comprised by residues 182-365 of BAP1 interact with the RING finger domain of BARD1, and surface plasmon resonance spectroscopy (BIAcore) analyses showed that BAP1 interferes with the BRCA1/BARD1 association. The perturbation resulted in inhibition of BRCA1 autoubiquitination and NPM1/B23 ubiquitination by BRCA1/BARD1. Although BAP1 was capable of deubiquitinating the polyubiquitin chains mediated by BRCA1/BARD1 in vitro, a catalytically inactive mutant of BAP1, C91S, still inhibited the ubiquitination in vitro and in vivo, implicating a second mechanism of action. Importantly, inhibition of BAP1 expression by short hairpin RNA resulted in hypersensitivity of the cells to ionizing irradiation and in retardation of S-phase progression. Together, these results suggest that BAP1 and BRCA1/BARD1 coordinately regulate ubiquitination during the DNA damage response and the cell cycle.

Pubmed ID: 19117993


  • Nishikawa H
  • Wu W
  • Koike A
  • Kojima R
  • Gomi H
  • Fukuda M
  • Ohta T


Cancer research

Publication Data

January 1, 2009

Associated Grants


Mesh Terms

  • BRCA1 Protein
  • Cell Line, Transformed
  • Down-Regulation
  • HeLa Cells
  • Humans
  • Infrared Rays
  • RNA, Small Interfering
  • S Phase
  • Tumor Suppressor Proteins
  • Ubiquitin
  • Ubiquitin Thiolesterase
  • Ubiquitin-Protein Ligases