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Hoxb5b acts downstream of retinoic acid signaling in the forelimb field to restrict heart field potential in zebrafish.

How adjacent organ fields communicate during development is not understood. Here, we identify a mechanism in which signaling within the forelimb field restricts the potential of the neighboring heart field. In zebrafish embryos deficient in retinoic acid (RA) signaling, the pectoral fins (forelimbs) are lost while both chambers of the heart are enlarged. We provide evidence that both of these phenotypes are due to RA signaling acting directly within the forelimb field. hoxb5b, an RA-responsive gene expressed within the forelimb field, is required to restrict the number of atrial cells arising from the adjacent heart field, although its function is dispensable for forelimb formation. Together, these data indicate nonautonomous influences downstream of RA signaling that act to limit individual chamber size. Therefore, our results offer new perspectives on the mechanisms regulating organ size and the possible causes of congenital syndromes affecting both the heart and forelimb.

Pubmed ID: 19081079

Authors

  • Waxman JS
  • Keegan BR
  • Roberts RW
  • Poss KD
  • Yelon D

Journal

Developmental cell

Publication Data

December 16, 2008

Associated Grants

  • Agency: NHLBI NIH HHS, Id: F32 HL083591
  • Agency: NHLBI NIH HHS, Id: F32 HL083591
  • Agency: NHLBI NIH HHS, Id: F32 HL083591-01
  • Agency: NHLBI NIH HHS, Id: F32 HL083591-02
  • Agency: NHLBI NIH HHS, Id: K99 HL091126
  • Agency: NHLBI NIH HHS, Id: K99 HL091126
  • Agency: NHLBI NIH HHS, Id: K99 HL091126-01
  • Agency: NHLBI NIH HHS, Id: R01 HL069594
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-01
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-02
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-03
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-04
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-05A1
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-06
  • Agency: NHLBI NIH HHS, Id: R01 HL069594-07
  • Agency: NHLBI NIH HHS, Id: R01 HL081674
  • Agency: NHLBI NIH HHS, Id: R01 HL081674-03
  • Agency: NIGMS NIH HHS, Id: T32 GM007308
  • Agency: NICHD NIH HHS, Id: T32 HD007520

Mesh Terms

  • Animals
  • Apoptosis
  • Cell Lineage
  • Forelimb
  • Gene Expression Regulation, Developmental
  • Heart
  • Heart Atria
  • Heart Ventricles
  • Homeodomain Proteins
  • Models, Biological
  • Phenotype
  • Signal Transduction
  • Time Factors
  • Tretinoin
  • Zebrafish
  • Zebrafish Proteins