• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Aurora-A kinase is essential for bipolar spindle formation and early development.

Aurora-A is a conserved kinase implicated in mitotic regulation and carcinogenesis. Aurora-A was previously implicated in mitotic entry and spindle assembly, although contradictory results prevented a clear understanding of the roles of Aurora-A in mammals. We developed a conditional null mutation in the mouse Aurora-A gene to investigate Aurora-A functions in primary cells ex vivo and in vivo. We show here that conditional Aurora-A ablation in cultured embryonic fibroblasts causes impaired mitotic entry and mitotic arrest with a profound defect in bipolar spindle formation. Germ line Aurora-A deficiency causes embryonic death at the blastocyst stage with pronounced cell proliferation failure, mitotic arrest, and monopolar spindle formation. Aurora-A deletion in mid-gestation embryos causes an increase in mitotic and apoptotic cells. These results indicate that murine Aurora-A facilitates, but is not absolutely required for, mitotic entry in murine embryonic fibroblasts and is essential for centrosome separation and bipolar spindle formation in vitro and in vivo. Aurora-A deletion increases apoptosis, suggesting that molecular therapies targeting Aurora-A may be effective in inducing tumor cell apoptosis. Aurora-A conditional mutant mice provide a valuable system for further defining Aurora-A functions and for predicting effects of Aurora-A therapeutic intervention.

Pubmed ID: 19075002

Authors

  • Cowley DO
  • Rivera-PĂ©rez JA
  • Schliekelman M
  • He YJ
  • Oliver TG
  • Lu L
  • O'Quinn R
  • Salmon ED
  • Magnuson T
  • Van Dyke T

Journal

Molecular and cellular biology

Publication Data

February 28, 2009

Associated Grants

  • Agency: NCI NIH HHS, Id: 2-R01-CA065773-11A1
  • Agency: NIGMS NIH HHS, Id: GM24364
  • Agency: NCI NIH HHS, Id: R01 CA065773
  • Agency: NCI NIH HHS, Id: R01 CA065773-11A1
  • Agency: NIGMS NIH HHS, Id: R01 GM024364
  • Agency: NIGMS NIH HHS, Id: R01 GM024364-21
  • Agency: NICHD NIH HHS, Id: R37 HD024462
  • Agency: NICHD NIH HHS, Id: R37 HD024462-11

Mesh Terms

  • Alleles
  • Animals
  • Apoptosis
  • Aurora Kinase A
  • Aurora Kinases
  • Blastocyst
  • Cell Proliferation
  • Embryo Loss
  • Embryo, Mammalian
  • Embryonic Development
  • Female
  • Fibroblasts
  • Gene Deletion
  • Gene Targeting
  • Mice
  • Mitosis
  • Mutation
  • Ploidies
  • Pregnancy
  • Protein-Serine-Threonine Kinases
  • Spindle Apparatus