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Down syndrome (DS) is a neurological disorder causing impaired learning and memory. Partial trisomy 16 mice (Ts65Dn) are a genetic model for DS. Previously, we demonstrated widespread alterations of pre- and postsynaptic elements and physiological abnormalities in Ts65Dn mice. The average diameter of presynaptic boutons and spines in the neocortex and hippocampus was enlarged. Failed induction of long-term potentiation (LTP) due to excessive inhibition was observed. In this paper we investigate the morphological substrate for excessive inhibition in Ts65Dn. We used electron microscopy (EM) to characterize synapses, confocal microscopy to analyze colocalization of the general marker for synaptic vesicle protein with specific protein markers for inhibitory and excitatory synapses, and densitometry to characterize the distribution of the receptor and several proteins essential for synaptic clustering of neurotransmitter receptors. EM analysis of synapses in the Ts65Dn vs. 2N showed that synaptic opposition lengths were significantly greater for symmetric synapses (approximately 18%), but not for asymmetric ones. Overall, a significant increase in colocalization coefficients of glutamic acid decarboxylase (GAD)65/p38 immunoreactivity (IR) (approximately 27%) and vesicular GABA transporter (VGAT)/p38 IR (approximately 41%) was found, but not in vesicular glutamate transporter 1 (VGLUT1)/p38 IR. A significant overall decrease of IR in the hippocampus of Ts65Dn mice compared with 2N mice for glutamate receptor 2 (GluR2; approximately 13%) and anti-gamma-aminobutyric acid (GABA)(A) receptor beta2/3 subunit (approximately 20%) was also found. The study of proteins essential for synaptic clustering of receptors revealed a significant increase in puncta size for neuroligin 2 (approximately 13%) and GABA(A) receptor-associated protein (GABARAP; approximately 13%), but not for neuroligin 1 and gephyrin. The results demonstrate a significant alteration of inhibitory synapses in the fascia dentata of Ts65Dn mice.
Pubmed ID: 19034952 RIS Download
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This polyclonal targets Gad2
View all literature mentionsThis polyclonal targets Glutamate receptor 1
View all literature mentionsThis monoclonal targets Glutamate Receptor 2 (GluR2), aa175-430
View all literature mentionsThis polyclonal targets NLGN2
View all literature mentionsThis unknown targets
View all literature mentionsThis polyclonal targets GABARAP antibody
View all literature mentionsThis monoclonal targets GABA A Receptor beta2/3 clone 62-3G1
View all literature mentionsThis polyclonal targets GABA A Receptor alpha 1
View all literature mentionsThis monoclonal targets Gephyrin
View all literature mentionsThis monoclonal targets Neuroligin 1/2/3/4
View all literature mentionsThis polyclonal targets GABA B Receptor 2 (GABABR2)
View all literature mentionsThis polyclonal targets GABA B Receptor R1
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