• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


Core structure of the yeast spt4-spt5 complex: a conserved module for regulation of transcription elongation.

The Spt4-Spt5 complex is an essential RNA polymerase II elongation factor found in all eukaryotes and important for gene regulation. We report here the crystal structure of Saccharomyces cerevisiae Spt4 bound to the NGN domain of Spt5. This structure reveals that Spt4-Spt5 binding is governed by an acid-dipole interaction between Spt5 and Spt4. Mutations that disrupt this interaction disrupt the complex. Residues forming this pivotal interaction are conserved in the archaeal homologs of Spt4 and Spt5, which we show also form a complex. Even though bacteria lack a Spt4 homolog, the NGN domains of Spt5 and its bacterial homologs are structurally similar. Spt4 is located at a position that may help to maintain the functional conformation of the following KOW domains in Spt5. This structural and evolutionary perspective of the Spt4-Spt5 complex and its homologs suggest that it is an ancient, core component of the transcription elongation machinery.

Pubmed ID: 19000817


  • Guo M
  • Xu F
  • Yamada J
  • Egelhofer T
  • Gao Y
  • Hartzog GA
  • Teng M
  • Niu L


Structure (London, England : 1993)

Publication Data

November 12, 2008

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM060479
  • Agency: NIGMS NIH HHS, Id: R01 GM060479-09

Mesh Terms

  • Amino Acid Sequence
  • Archaea
  • Archaeal Proteins
  • Chromosomal Proteins, Non-Histone
  • Conserved Sequence
  • Crystallography, X-Ray
  • Models, Molecular
  • Molecular Sequence Data
  • Nuclear Proteins
  • Protein Binding
  • Protein Conformation
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Sequence Alignment
  • Transcription, Genetic
  • Transcriptional Elongation Factors