Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Histone H3 K36 methylation is mediated by a trans-histone methylation pathway involving an interaction between Set2 and histone H4.

Genes & development | Oct 15, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18923077

Set2-mediated H3 K36 methylation is an important histone modification on chromatin during transcription elongation. Although Set2 associates with the phosphorylated C-terminal domain (CTD) of RNA polymerase II (RNAPII), the mechanism of Set2 binding to chromatin and subsequent exertion of its methyltransferase activity is relatively uncharacterized. We identified a critical lysine residue in histone H4 that is needed for interaction with Set2 and proper H3 K36 di- and trimethylation. We also determined that the N terminus of Set2 contains a histone H4 interaction motif that allows Set2 to bind histone H4 and nucleosomes. A Set2 mutant lacking the histone H4 interaction motif is able to bind to the phosphorylated CTD of RNAPII and associate with gene-specific loci but is defective for H3 K36 di- and trimethylation. In addition, this Set2 mutant shows increased H4 acetylation and resistance to 6-Azauracil. Overall, our study defines a new interaction between Set2 and histone H4 that mediates trans-histone regulation of H3 K36 methylation, which is needed for the preventative maintenance and integrity of the genome.

Pubmed ID: 18923077 RIS Download

Mesh terms: Antimetabolites | Blotting, Western | Chromatin | Chromatin Immunoprecipitation | Gene Expression Regulation, Fungal | Histones | Immunoblotting | Immunoprecipitation | Lysine | Methylation | Methyltransferases | Mutation | Nucleosomes | Phosphorylation | Protein Kinases | Protein Processing, Post-Translational | RNA Polymerase II | Saccharomyces cerevisiae Proteins | Transcription, Genetic | Uracil

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: GM74183
  • Agency: NIGMS NIH HHS, Id: R01 GM074183
  • Agency: NIGMS NIH HHS, Id: R01 GM074183-01A1
  • Agency: NIGMS NIH HHS, Id: R01 GM074183-02
  • Agency: NIGMS NIH HHS, Id: R01 GM074183-03
  • Agency: NIGMS NIH HHS, Id: R01 GM074183-04

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.