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Hedgehog signaling plays a cell-autonomous role in maximizing cardiac developmental potential.

Elucidation of the complete roster of signals required for myocardial specification is crucial to the future of cardiac regenerative medicine. Prior studies have implicated the Hedgehog (Hh) signaling pathway in the regulation of multiple aspects of heart development. However, our understanding of the contribution of Hh signaling to the initial specification of myocardial progenitor cells remains incomplete. Here, we show that Hh signaling promotes cardiomyocyte formation in zebrafish. Reduced Hh signaling creates a cardiomyocyte deficit, and increased Hh signaling creates a surplus. Through fate-mapping, we find that Hh signaling is required at early stages to ensure specification of the proper number of myocardial progenitors. Genetic inducible fate mapping in mouse indicates that myocardial progenitors respond directly to Hh signals, and transplantation experiments in zebrafish demonstrate that Hh signaling acts cell autonomously to promote the contribution of cells to the myocardium. Thus, Hh signaling plays an essential early role in defining the optimal number of cardiomyocytes, making it an attractive target for manipulation of multipotent progenitor cells.

Pubmed ID: 18842815

Authors

  • Thomas NA
  • Koudijs M
  • van Eeden FJ
  • Joyner AL
  • Yelon D

Journal

Development (Cambridge, England)

Publication Data

November 27, 2008

Associated Grants

  • Agency: NCI NIH HHS, Id: R01 CA128158
  • Agency: NICHD NIH HHS, Id: R01 HD035768
  • Agency: NICHD NIH HHS, Id: R01 HD35768
  • Agency: NHLBI NIH HHS, Id: R01 HL069594
  • Agency: NICHD NIH HHS, Id: T32 HD007520

Mesh Terms

  • Animals
  • Cell Differentiation
  • Cell Proliferation
  • Endothelial Cells
  • Gene Expression Regulation, Developmental
  • Heart
  • Hedgehog Proteins
  • Mice
  • Myocardium
  • Signal Transduction
  • Stem Cells
  • Zebrafish