• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

A protein domain-based interactome network for C. elegans early embryogenesis.

Many protein-protein interactions are mediated through independently folding modular domains. Proteome-wide efforts to model protein-protein interaction or "interactome" networks have largely ignored this modular organization of proteins. We developed an experimental strategy to efficiently identify interaction domains and generated a domain-based interactome network for proteins involved in C. elegans early-embryonic cell divisions. Minimal interacting regions were identified for over 200 proteins, providing important information on their domain organization. Furthermore, our approach increased the sensitivity of the two-hybrid system, resulting in a more complete interactome network. This interactome modeling strategy revealed insights into C. elegans centrosome function and is applicable to other biological processes in this and other organisms.

Pubmed ID: 18692475

Authors

  • Boxem M
  • Maliga Z
  • Klitgord N
  • Li N
  • Lemmens I
  • Mana M
  • de Lichtervelde L
  • Mul JD
  • van de Peut D
  • Devos M
  • Simonis N
  • Yildirim MA
  • Cokol M
  • Kao HL
  • de Smet AS
  • Wang H
  • Schlaitz AL
  • Hao T
  • Milstein S
  • Fan C
  • Tipsword M
  • Drew K
  • Galli M
  • Rhrissorrakrai K
  • Drechsel D
  • Koller D
  • Roth FP
  • Iakoucheva LM
  • Dunker AK
  • Bonneau R
  • Gunsalus KC
  • Hill DE
  • Piano F
  • Tavernier J
  • van den Heuvel S
  • Hyman AA
  • Vidal M

Journal

Cell

Publication Data

August 8, 2008

Associated Grants

  • Agency: NCI NIH HHS, Id: CA95281
  • Agency: NCI NIH HHS, Id: P01 CA095281-01A10004
  • Agency: NHGRI NIH HHS, Id: R01 HG001715
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-06
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-07
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-07S1
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-08
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-09
  • Agency: NHGRI NIH HHS, Id: R01 HG001715-10
  • Agency: NHGRI NIH HHS, Id: R01HG001715
  • Agency: NCI NIH HHS, Id: R21 CA113711
  • Agency: NCI NIH HHS, Id: R21 CA113711-01A2
  • Agency: NCRR NIH HHS, Id: R21 RR023114-01
  • Agency: NCRR NIH HHS, Id: R21 RR023114-02
  • Agency: NCI NIH HHS, Id: R21CA113711
  • Agency: NCRR NIH HHS, Id: R21RR023114
  • Agency: NCI NIH HHS, Id: R33 CA081658
  • Agency: NCI NIH HHS, Id: R33 CA081658-04
  • Agency: NCI NIH HHS, Id: R33 CA105405
  • Agency: NCI NIH HHS, Id: R33 CA105405-01
  • Agency: NCI NIH HHS, Id: R33 CA105405-02
  • Agency: NCI NIH HHS, Id: R33 CA105405-03
  • Agency: NCI NIH HHS, Id: R33CA105405
  • Agency: NCI NIH HHS, Id: R33CA81658
  • Agency: NCI NIH HHS, Id: U54 CA011295
  • Agency: NCI NIH HHS, Id: U54 CA112952
  • Agency: NCI NIH HHS, Id: U54 CA112952-01
  • Agency: NCI NIH HHS, Id: U54 CA112952-02
  • Agency: NCI NIH HHS, Id: U54 CA112952-03

Mesh Terms

  • Animals
  • Caenorhabditis elegans
  • Cell Division
  • Embryo, Nonmammalian
  • Embryonic Development
  • Protein Interaction Domains and Motifs
  • Protein Interaction Mapping
  • Proteome
  • Two-Hybrid System Techniques