Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Evi-1 is a critical regulator for hematopoietic stem cells and transformed leukemic cells.

Cell stem cell | Aug 7, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18682242

Evi-1 has been recognized as one of the dominant oncogenes associated with murine and human myeloid leukemia. Here, we show that hematopoietic stem cells (HSCs) in Evi-1-deficient embryos are severely reduced in number with defective proliferative and repopulating capacity. Selective ablation of Evi-1 in Tie2(+) cells mimics Evi-1 deficiency, suggesting that Evi-1 function is required in Tie2(+) hematopoietic stem/progenitors. Conditional deletion of Evi-1 in the adult hematopoietic system revealed that Evi-1-deficient bone marrow HSCs cannot maintain hematopoiesis and lose their repopulating ability. In contrast, Evi-1 is dispensable for blood cell lineage commitment. Evi-1(+/-) mice exhibit the intermediate phenotype for HSC activity, suggesting a gene dosage requirement for Evi-1. We further demonstrate that disruption of Evi-1 in transformed leukemic cells leads to significant loss of their proliferative activity both in vitro and in vivo. Thus, Evi-1 is a common and critical regulator essential for proliferation of embryonic/adult HSCs and transformed leukemic cells.

Pubmed ID: 18682242 RIS Download

Mesh terms: Animals | Bone Marrow Cells | Cell Line, Transformed | Cell Lineage | Cell Proliferation | Cell Survival | DNA-Binding Proteins | Gene Dosage | Hematopoiesis | Hematopoietic Stem Cell Transplantation | Hematopoietic Stem Cells | Humans | Leukemia, Myeloid | Mice | Mice, Inbred C57BL | Mice, Knockout | Microarray Analysis | Proto-Oncogenes | Receptor, TIE-2 | Transcription Factors

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

None

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.