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Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.

Science (New York, N.Y.) | Jul 25, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18653893

Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.

Pubmed ID: 18653893 RIS Download

Mesh terms: Acetylation | Acetyltransferases | Alleles | Cell Cycle Proteins | Chondroitin Sulfate Proteoglycans | Chromatids | Chromosomal Proteins, Non-Histone | Chromosomes, Fungal | DNA Repair | DNA Replication | DNA, Fungal | Mutation | Nuclear Proteins | Protein Subunits | S Phase | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Suppression, Genetic

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