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Eco1-dependent cohesin acetylation during establishment of sister chromatid cohesion.

Replicated chromosomes are held together by the chromosomal cohesin complex from the time of their synthesis in S phase onward. This requires the replication fork-associated acetyl transferase Eco1, but Eco1's mechanism of action is not known. We identified spontaneous suppressors of the thermosensitive eco1-1 allele in budding yeast. An acetylation-mimicking mutation of a conserved lysine in cohesin's Smc3 subunit makes Eco1 dispensable for cell growth, and we show that Smc3 is acetylated in an Eco1-dependent manner during DNA replication to promote sister chromatid cohesion. A second set of eco1-1 suppressors inactivate the budding yeast ortholog of the cohesin destabilizer Wapl. Our results indicate that Eco1 modifies cohesin to stabilize sister chromatid cohesion in parallel with a cohesion establishment reaction that is in principle Eco1-independent.

Pubmed ID: 18653893

Authors

  • Rolef Ben-Shahar T
  • Heeger S
  • Lehane C
  • East P
  • Flynn H
  • Skehel M
  • Uhlmann F

Journal

Science (New York, N.Y.)

Publication Data

July 25, 2008

Associated Grants

None

Mesh Terms

  • Acetylation
  • Acetyltransferases
  • Alleles
  • Cell Cycle Proteins
  • Chondroitin Sulfate Proteoglycans
  • Chromatids
  • Chromosomal Proteins, Non-Histone
  • Chromosomes, Fungal
  • DNA Repair
  • DNA Replication
  • DNA, Fungal
  • Mutation
  • Nuclear Proteins
  • Protein Subunits
  • S Phase
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Suppression, Genetic