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Syntaxin 1A interaction with the dopamine transporter promotes amphetamine-induced dopamine efflux.

Molecular pharmacology | Oct 19, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18617632

The soluble N-ethylmaleimide-sensitive factor attachment protein receptor protein syntaxin 1A (SYN1A) interacts with and regulates the function of transmembrane proteins, including ion channels and neurotransmitter transporters. Here, we define the first 33 amino acids of the N terminus of the dopamine (DA) transporter (DAT) as the site of direct interaction with SYN1A. Amphetamine (AMPH) increases the association of SYN1A with human DAT (hDAT) in a heterologous expression system (hDAT cells) and with native DAT in murine striatal synaptosomes. Immunoprecipitation of DAT from the biotinylated fraction shows that the AMPH-induced increase in DAT/SYN1A association occurs at the plasma membrane. In a superfusion assay of DA efflux, cells overexpressing SYN1A exhibited significantly greater AMPH-induced DA release with respect to control cells. By combining the patch-clamp technique with amperometry, we measured DA release under voltage clamp. At -60 mV, a physiological resting potential, AMPH did not induce DA efflux in hDAT cells and DA neurons. In contrast, perfusion of exogenous SYN1A (3 microM) into the cell with the whole-cell pipette enabled AMPH-induced DA efflux at -60 mV in both hDAT cells and DA neurons. It has been shown recently that Ca2+/calmodulin-dependent protein kinase II (CaMKII) is activated by AMPH and regulates AMPH-induced DA efflux. Here, we show that AMPH-induced association between DAT and SYN1A requires CaMKII activity and that inhibition of CaMKII blocks the ability of exogenous SYN1A to promote DA efflux. These data suggest that AMPH activation of CaMKII supports DAT/SYN1A association, resulting in a mode of DAT capable of DA efflux.

Pubmed ID: 18617632 RIS Download

Mesh terms: Amino Acid Sequence | Amphetamine | Animals | Cell Line | Cell Membrane | Cells, Cultured | Corpus Striatum | Dopamine | Dopamine Plasma Membrane Transport Proteins | Glutathione Transferase | Humans | Kidney | Mesencephalon | Mice | Mice, Transgenic | Molecular Sequence Data | Neurons | Recombinant Fusion Proteins | Synaptosomes | Syntaxin 1 | Transfection

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Associated grants

  • Agency: NIDA NIH HHS, Id: DA011697
  • Agency: NIDA NIH HHS, Id: DA012408
  • Agency: NIDA NIH HHS, Id: DA022413
  • Agency: NIDA NIH HHS, Id: DA13975
  • Agency: NIDA NIH HHS, Id: F31 DA021069-02
  • Agency: NIMH NIH HHS, Id: F31 MH081423-02
  • Agency: NIDA NIH HHS, Id: F31-DA021069
  • Agency: NIMH NIH HHS, Id: F31-MH081423
  • Agency: NIDA NIH HHS, Id: F32 DA020306
  • Agency: NIDA NIH HHS, Id: F32 DA020306-03
  • Agency: NIDA NIH HHS, Id: F32-DA020306
  • Agency: NIDA NIH HHS, Id: K05 DA022413
  • Agency: NIDA NIH HHS, Id: K05 DA022413-02
  • Agency: NIMH NIH HHS, Id: MH058921
  • Agency: NIDA NIH HHS, Id: P01 DA012408
  • Agency: NIDA NIH HHS, Id: P01 DA012408-100003
  • Agency: NIDA NIH HHS, Id: P01 DA012408-100004
  • Agency: NIDA NIH HHS, Id: P01 DA012408-109001
  • Agency: NIDA NIH HHS, Id: R01 DA011697
  • Agency: NIDA NIH HHS, Id: R01 DA011697-08
  • Agency: NIDA NIH HHS, Id: R01 DA013975
  • Agency: NIDA NIH HHS, Id: R01 DA013975-09
  • Agency: NIMH NIH HHS, Id: R01 MH054137
  • Agency: NIMH NIH HHS, Id: R01 MH054137-13
  • Agency: NIMH NIH HHS, Id: R01 MH063232
  • Agency: NIMH NIH HHS, Id: R01 MH063232-08
  • Agency: NIMH NIH HHS, Id: T32 MH018870

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