We have updated our privacy policy. If you have any question, contact us at privacy@scicrunch.org. Dismiss and don't show again

Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The meiosis-specific Sid2p-related protein Slk1p regulates forespore membrane assembly in fission yeast.

Cytokinesis in all organisms involves the creation of membranous barriers that demarcate individual daughter cells. In fission yeast, a signaling module termed the septation initiation network (SIN) plays an essential role in the assembly of new membranes and cell wall during cytokinesis. In this study, we have characterized Slk1p, a protein-kinase related to the SIN component Sid2p. Slk1p is expressed specifically during meiosis and localizes to the spindle pole bodies (SPBs) during meiosis I and II in a SIN-dependent manner. Slk1p also localizes to the forespore membrane during sporulation. Cells lacking Slk1p display defects associated with sporulation, leading frequently to the formation of asci with smaller and/or fewer spores. The ability of slk1 Delta cells to sporulate, albeit inefficiently, is fully abolished upon compromise of function of Sid2p, suggesting that Slk1p and Sid2p play overlapping roles in sporulation. Interestingly, increased expression of the syntaxin Psy1p rescues the sporulation defect of sid2-250 slk1 Delta. Thus, it is likely that Slk1p and Sid2p play a role in forespore membrane assembly by facilitating recruitment of components of the secretory apparatus, such as Psy1p, to allow membrane expansion. These studies thereby provide a novel link between the SIN and vesicle trafficking during cytokinesis.

Pubmed ID: 18562696 RIS Download

Mesh terms: Amino Acid Sequence | Cell Membrane | Cytokinesis | Gene Expression Regulation, Enzymologic | Gene Expression Regulation, Fungal | Green Fluorescent Proteins | Meiosis | Mitogen-Activated Protein Kinase Kinases | Models, Biological | Molecular Sequence Data | Protein Kinases | Qa-SNARE Proteins | Saccharomyces cerevisiae Proteins | Schizosaccharomyces | Schizosaccharomyces pombe Proteins | Sequence Homology, Amino Acid | Signal Transduction

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM058406
  • Agency: NIGMS NIH HHS, Id: R01 GM062184
  • Agency: NIGMS NIH HHS, Id: GM058406
  • Agency: NIGMS NIH HHS, Id: GM62184

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.