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The study of adult stem cells relies on the ability to isolate them using complex combinations of markers for flow cytometry. A recent study has used a tetracycline-regulatable H2B-GFP transgenic mouse model analogous to BrdU pulse-chase methods to fluorescently label quiescent skin stem cells in vivo. In this study, we sought to use these mice to fluorescently label hematopoietic stem cells to study niche interactions.
Pubmed ID: 18523660 RIS Download
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Mus musculus with name B6.SJL-Ptprca Pepc
Mus musculus with name C57BL/6N-Tg(CAG-H2b/GFP)/MarpApb from IMSR.
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