Searching across hundreds of databases

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Optimized lentiviral transduction of mouse bone marrow-derived mesenchymal stem cells.

Mesenchymal stem cells (MSCs) have attracted much attention as potential platforms for transgene delivery and cell-based therapy for human disease. MSCs have the capability to self-renew and retain multipotency after extensive expansion in vitro, making them attractive targets for ex vivo modification and autologous transplantation. Viral vectors, including lentiviral vectors, provide an efficient means for transgene delivery into human MSCs. In contrast, mouse MSCs have proven more difficult to transduce with lentiviral vectors than their human counterparts, and because many studies use mouse models of human disease, an improved method of transduction would facilitate studies using ex vivo-modified mouse MSCs. We have worked toward improving the production of human immunodeficiency virus type 1 (HIV-1)-based lentiviral vectors and optimizing transduction conditions for mouse MSCs using lentivirus vectors pseudotyped with the vesicular stomatitis virus G glycoprotein (VSV-G), the ecotropic murine leukemia virus envelope glycoprotein (MLV-E), and the glycoproteins derived from the Armstrong and WE strains of lymphocytic choriomeningitis virus (LCMV-Arm, LCMV-WE). Mouse MSCs were readily transduced following overnight incubation using a multiplicity of infection of at least 40. Alternatively, mouse MSCs in suspension were readily transduced after a 1-h exposure to lentiviral pseudotypes immediately following trypsin treatment or retrieval from storage in liquid nitrogen. LCMV-WE pseudotypes resulted in efficient transduction of mouse MSCs with less toxicity than VSV-G pseudotypes. In conclusion, our improved production and transduction conditions for lentiviral vectors resulted in efficient transduction of mouse MSCs, and these improvements should facilitate the application of such cells in the context of mouse models of human disease.

Pubmed ID: 18513160 RIS Download

Mesh terms: Animals | Antigens, CD | Antigens, CD9 | Bone Marrow Cells | Cell Proliferation | Flow Cytometry | Genetic Vectors | Glycoproteins | Humans | Lentivirus | Lipids | Membrane Glycoproteins | Mesenchymal Stromal Cells | Mice | Mice, Inbred C57BL | Receptors, Interleukin-8B | Time Factors | Transduction, Genetic

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NHLBI NIH HHS, Id: R01 HL073770
  • Agency: NCRR NIH HHS, Id: P40RR017447
  • Agency: NINDS NIH HHS, Id: NS044832
  • Agency: NHLBI NIH HHS, Id: R01 HL073770-04
  • Agency: NHLBI NIH HHS, Id: R01 HL073770-03
  • Agency: NHLBI NIH HHS, Id: R01 HL073770-04S1
  • Agency: NHLBI NIH HHS, Id: R01 HL073770-02
  • Agency: NHLBI NIH HHS, Id: R01 HL073770-01
  • Agency: NHLBI NIH HHS, Id: HL075161
  • Agency: NHLBI NIH HHS, Id: HL073770

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.

We have not found any resources mentioned in this publication.