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The epithelial-mesenchymal transition generates cells with properties of stem cells.

The epithelial-mesenchymal transition (EMT) is a key developmental program that is often activated during cancer invasion and metastasis. We here report that the induction of an EMT in immortalized human mammary epithelial cells (HMLEs) results in the acquisition of mesenchymal traits and in the expression of stem-cell markers. Furthermore, we show that those cells have an increased ability to form mammospheres, a property associated with mammary epithelial stem cells. Independent of this, stem cell-like cells isolated from HMLE cultures form mammospheres and express markers similar to those of HMLEs that have undergone an EMT. Moreover, stem-like cells isolated either from mouse or human mammary glands or mammary carcinomas express EMT markers. Finally, transformed human mammary epithelial cells that have undergone an EMT form mammospheres, soft agar colonies, and tumors more efficiently. These findings illustrate a direct link between the EMT and the gain of epithelial stem cell properties.

Pubmed ID: 18485877

Authors

  • Mani SA
  • Guo W
  • Liao MJ
  • Eaton EN
  • Ayyanan A
  • Zhou AY
  • Brooks M
  • Reinhard F
  • Zhang CC
  • Shipitsin M
  • Campbell LL
  • Polyak K
  • Brisken C
  • Yang J
  • Weinberg RA

Journal

Cell

Publication Data

May 16, 2008

Associated Grants

  • Agency: NCI NIH HHS, Id: CA89393
  • Agency: NCI NIH HHS, Id: CA94074
  • Agency: NCI NIH HHS, Id: P0-CA080111
  • Agency: NCI NIH HHS, Id: P01 CA080111
  • Agency: NCI NIH HHS, Id: P01 CA080111-09
  • Agency: NCI NIH HHS, Id: P50 CA089393
  • Agency: NCI NIH HHS, Id: P50 CA089393-060014
  • Agency: NCI NIH HHS, Id: P50 CA089393-070014
  • Agency: NCI NIH HHS, Id: P50 CA089393-080014
  • Agency: NCI NIH HHS, Id: R01 CA078461
  • Agency: NCI NIH HHS, Id: R01 CA078461-09
  • Agency: NCI NIH HHS, Id: R01 CA116235
  • Agency: NCI NIH HHS, Id: R01 CA116235-01A1
  • Agency: NCI NIH HHS, Id: R01 CA116235-02
  • Agency: NCI NIH HHS, Id: R01 CA116235-03
  • Agency: NCI NIH HHS, Id: R01-CA078461

Mesh Terms

  • Adult Stem Cells
  • Animals
  • Antigens, CD24
  • Antigens, CD44
  • Cell Transformation, Neoplastic
  • Cells, Cultured
  • Epithelial Cells
  • Humans
  • Mammary Glands, Animal
  • Mammary Glands, Human
  • Mesoderm
  • Mice
  • Neoplastic Stem Cells
  • Spheroids, Cellular
  • Stem Cells
  • Tumor Cells, Cultured