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Monovalent, reduced-size quantum dots for imaging receptors on living cells.

Nature methods | May 30, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18425138

We describe a method to generate monovalent quantum dots (QDs) using agarose gel electrophoresis. We passivated QDs with a carboxy-terminated polyethylene-glycol ligand, yielding particles with half the diameter of commercial QDs, which we conjugated to a single copy of a high-affinity targeting moiety (monovalent streptavidin or antibody to carcinoembryonic antigen) to label cell-surface proteins. The small size improved access of QD-labeled glutamate receptors to neuronal synapses, and monovalency prevented EphA3 tyrosine kinase activation.

Pubmed ID: 18425138 RIS Download

Mesh terms: Animals | Carcinoembryonic Antigen | Cell Survival | Cells | Cells, Cultured | Electrophoresis, Agar Gel | Fluorescent Dyes | Image Processing, Computer-Assisted | Ligands | Nanotechnology | Polyethylene Glycols | Quantum Dots | Rats | Receptors, Cell Surface | Receptors, Glutamate | Staining and Labeling | Streptavidin | Synapses

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Associated grants

  • Agency: NCI NIH HHS, Id: CA101830
  • Agency: NIGMS NIH HHS, Id: P20GM072029-01
  • Agency: NIGMS NIH HHS, Id: R01 GM072670
  • Agency: NIGMS NIH HHS, Id: R01 GM072670-01
  • Agency: NIGMS NIH HHS, Id: T32 GM008334

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