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Reconstitution of contractile FtsZ rings in liposomes.

Science (New York, N.Y.) | May 9, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18420899

FtsZ is a tubulin homolog and the major cytoskeletal protein in bacterial cell division. It assembles into the Z ring, which contains FtsZ and a dozen other division proteins, and constricts to divide the cell. We have constructed a membrane-targeted FtsZ (FtsZ-mts) by splicing an amphipathic helix to its C terminus. When mixed with lipid vesicles, FtsZ-mts was incorporated into the interior of some tubular vesicles. There it formed multiple Z rings that could move laterally in both directions along the length of the liposome and coalesce into brighter Z rings. Brighter Z rings produced visible constrictions in the liposome, suggesting that FtsZ itself can assemble the Z ring and generate a force. No other proteins were needed for assembly and force generation.

Pubmed ID: 18420899 RIS Download

Mesh terms: Bacterial Proteins | Cell Membrane | Cytoskeletal Proteins | Escherichia coli | Escherichia coli Proteins | Liposomes | Membrane Proteins | Protein Binding | Protein Transport | Recombinant Proteins