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Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control.

Molecular cell | Apr 11, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18406327

Histone deacetylases (HDACs) are enzymes that modify key residues in histones to regulate chromatin architecture, and they play a vital role in cell survival, cell-cycle progression, and tumorigenesis. To understand the function of Hdac3, a critical component of the N-CoR/SMRT repression complex, a conditional allele of Hdac3 was engineered. Cre-recombinase-mediated inactivation of Hdac3 led to a delay in cell-cycle progression, cell-cycle-dependent DNA damage, and apoptosis in mouse embryonic fibroblasts (MEFs). While no overt defects in mitosis were observed in Hdac3-/- MEFs, including normal H3Ser10 phosphorylation, DNA damage was observed in Hdac3-/- interphase cells, which appears to be associated with defective DNA double-strand break repair. Moreover, we noted that Hdac3-/- MEFs were protected from DNA damage when quiescent, which may provide a mechanistic basis for the action of HDAC inhibitors on cycling tumor cells.

Pubmed ID: 18406327 RIS Download

Mesh terms: Animals | Apoptosis | Caffeine | Cells, Cultured | DNA Damage | DNA Repair | Fibroblasts | Gene Expression Profiling | Gene Expression Regulation | Histone Deacetylases | Humans | Mice | Mice, Knockout | Mitosis | NIH 3T3 Cells | Neoplasms | Oligonucleotide Array Sequence Analysis | Phenotype | Phosphodiesterase Inhibitors | Radiation, Ionizing | S Phase

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Associated grants

  • Agency: NCI NIH HHS, Id: P30-CA68485
  • Agency: NIDDK NIH HHS, Id: P30DK58404
  • Agency: NCI NIH HHS, Id: R01 CA064140
  • Agency: NCI NIH HHS, Id: R01 CA064140-15
  • Agency: NCI NIH HHS, Id: R01 CA077274
  • Agency: NCI NIH HHS, Id: R01 CA077274-10
  • Agency: NCI NIH HHS, Id: R01 CA109355
  • Agency: NCI NIH HHS, Id: R01-CA64140
  • Agency: NCI NIH HHS, Id: R01-CA77274
  • Agency: NCI NIH HHS, Id: R21 CA132010
  • Agency: NCI NIH HHS, Id: R21 CA132010-02

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