Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Gli activity correlates with tumor grade in platelet-derived growth factor-induced gliomas.

Cancer research | Apr 1, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18381430

Gli signaling is critical for central nervous system development and is implicated in tumorigenesis. To monitor Gli signaling in gliomas in vivo, we created platelet-derived growth factor-induced gliomas in a Gli-luciferase reporter mouse. We find that Gli activation is found in gliomas and correlates with grade. In addition, we find that sonic hedgehog (SHH) is expressed in these tumors and also correlates with grade. We identify microvascular proliferation and pseudopalisades, elements that define high-grade gliomas as SHH-producing microenvironments. We describe two populations of SHH-producing stromal cells that reside in perivascular niche (PVN), namely low-cycling astrocytes and endothelial cells. Using the Ptc-LacZ knock-in mouse as a second Gli responsive reporter, we show beta-galactosidase activity in the PVN and in some tumors diffusely throughout the tumor. Lastly, we observe that SHH is similarly expressed in human gliomas and note that an intact tumor microenvironment or neurosphere conditions in vitro are required for Gli activity.

Pubmed ID: 18381430 RIS Download

Mesh terms: Animals | Astrocytes | Brain Neoplasms | Cell Line, Tumor | Down-Regulation | Genes, Reporter | Glioblastoma | Glioma | Hedgehog Proteins | Humans | Kruppel-Like Transcription Factors | Mice | Mice, Transgenic | Oligodendroglioma | Platelet-Derived Growth Factor | Transcription Factors | beta-Galactosidase

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.