Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Genetic control of neuronal activity in mice conditionally expressing TRPV1.

Nature methods | Apr 31, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18327266

Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.

Pubmed ID: 18327266 RIS Download

Mesh terms: Action Potentials | Animals | Behavior, Animal | Brain | Capsaicin | Gene Targeting | Genes, Reporter | Genetic Vectors | Green Fluorescent Proteins | Immunohistochemistry | Integrases | Lentivirus | Mice | Mice, Knockout | Neurons | Patch-Clamp Techniques | Rats | Recombination, Genetic | TRPV Cation Channels | Transfection

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NINDS NIH HHS, Id: K99 NS064171
  • Agency: NINDS NIH HHS, Id: R01 NS047574
  • Agency: NINDS NIH HHS, Id: R01 NS047574-07
  • Agency: Howard Hughes Medical Institute, Id:

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.