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Genetic control of neuronal activity in mice conditionally expressing TRPV1.

Here we describe a knock-in mouse model for Cre-loxP-based conditional expression of TRPV1 in central nervous system neurons. Expression of Cre recombinase using biolistics, lentivirus or genetic intercrosses triggered heterologous expression of TRPV1 in a cell-specific manner. Application of the TRPV1 ligand capsaicin induced strong inward currents, triggered action potentials and activated stereotyped behaviors, allowing cell type-specific chemical genetic control of neuronal activity in vitro and in vivo.

Pubmed ID: 18327266

Authors

  • Arenkiel BR
  • Klein ME
  • Davison IG
  • Katz LC
  • Ehlers MD

Journal

Nature methods

Publication Data

April 31, 2008

Associated Grants

  • Agency: NINDS NIH HHS, Id: K99 NS064171
  • Agency: NINDS NIH HHS, Id: R01 NS047574
  • Agency: NINDS NIH HHS, Id: R01 NS047574-07
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Action Potentials
  • Animals
  • Behavior, Animal
  • Brain
  • Capsaicin
  • Gene Targeting
  • Genes, Reporter
  • Genetic Vectors
  • Green Fluorescent Proteins
  • Immunohistochemistry
  • Integrases
  • Lentivirus
  • Mice
  • Mice, Knockout
  • Neurons
  • Patch-Clamp Techniques
  • Rats
  • Recombination, Genetic
  • TRPV Cation Channels
  • Transfection