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NADP regulates the yeast GAL induction system.

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

Pubmed ID: 18292341

Authors

  • Kumar PR
  • Yu Y
  • Sternglanz R
  • Johnston SA
  • Joshua-Tor L

Journal

Science (New York, N.Y.)

Publication Data

February 22, 2008

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM074075
  • Agency: NIGMS NIH HHS, Id: GM55641
  • Agency: NCI NIH HHS, Id: P30 CA045508
  • Agency: NIGMS NIH HHS, Id: R01 GM074075
  • Agency: NIGMS NIH HHS, Id: R01 GM074075-04
  • Agency: Howard Hughes Medical Institute, Id:

Mesh Terms

  • Amino Acid Motifs
  • Binding Sites
  • Crystallography, X-Ray
  • DNA-Binding Proteins
  • Dimerization
  • Galactokinase
  • Galactose
  • Gene Expression Regulation, Fungal
  • Models, Molecular
  • NADP
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Recombinant Fusion Proteins
  • Repressor Proteins
  • Saccharomyces cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • Transcription, Genetic