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NADP regulates the yeast GAL induction system.

Science (New York, N.Y.) | Feb 22, 2008

Transcriptional regulation of the galactose-metabolizing genes in Saccharomyces cerevisiae depends on three core proteins: Gal4p, the transcriptional activator that binds to upstream activating DNA sequences (UAS(GAL)); Gal80p, a repressor that binds to the carboxyl terminus of Gal4p and inhibits transcription; and Gal3p, a cytoplasmic transducer that, upon binding galactose and adenosine 5'-triphosphate, relieves Gal80p repression. The current model of induction relies on Gal3p sequestering Gal80p in the cytoplasm. However, the rapid induction of this system implies that there is a missing factor. Our structure of Gal80p in complex with a peptide from the carboxyl-terminal activation domain of Gal4p reveals the existence of a dinucleotide that mediates the interaction between the two. Biochemical and in vivo experiments suggests that nicotinamide adenine dinucleotide phosphate (NADP) plays a key role in the initial induction event.

Pubmed ID: 18292341 RIS Download

Mesh terms: Amino Acid Motifs | Binding Sites | Crystallography, X-Ray | DNA-Binding Proteins | Dimerization | Galactokinase | Galactose | Gene Expression Regulation, Fungal | Models, Molecular | NADP | Protein Structure, Secondary | Protein Structure, Tertiary | Recombinant Fusion Proteins | Repressor Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription Factors | Transcription, Genetic

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Associated grants

  • Agency: Howard Hughes Medical Institute, Id: HHMI_JOSHUA-TOR_L
  • Agency: NIGMS NIH HHS, Id: R01 GM074075
  • Agency: NIGMS NIH HHS, Id: GM074075
  • Agency: NIGMS NIH HHS, Id: GM55641
  • Agency: NCI NIH HHS, Id: P30 CA045508
  • Agency: NIGMS NIH HHS, Id: R01 GM074075-04

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