• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Tandem immunoprecipitation of phosphotyrosine-mass spectrometry (TIPY-MS) indicates C19ORF19 becomes tyrosine-phosphorylated and associated with activated epidermal growth factor receptor.

To identify phosphotyrosine (pY) sites in the epidermal growth factor receptor (EGFR) network, a tandem immunoprecipitation-mass spectrometry method (TIPY-MS) was applied wherein protease-digested EGFR immune complexes were extracted with anti-pY after Rush et al. ( Nat. Biotech. 2005, 23, 94 ) and analyzed by LC-MS/MS. New pY sites in the pathway were found, including SOS1 Y1065, SOS2 Y1275, CBL-B Y889, and in the EGFR regulatory protein Mig-6 Y458. The novel human C19orf19 gene product was found EGFR-associated and phosphorylated at 5 tyrosines in response to EGFR activation and, therefore, represents a new component of the EGFR signaling network.

Pubmed ID: 18271526

Authors

  • Tong J
  • Taylor P
  • Jovceva E
  • St-Germain JR
  • Jin LL
  • Nikolic A
  • Gu X
  • Li ZH
  • Trudel S
  • Moran MF

Journal

Journal of proteome research

Publication Data

March 10, 2008

Associated Grants

None

Mesh Terms

  • Amino Acid Sequence
  • Cell Line
  • Humans
  • Immunoprecipitation
  • Mass Spectrometry
  • Molecular Sequence Data
  • Open Reading Frames
  • Phosphorylation
  • Phosphotyrosine
  • Receptor, Epidermal Growth Factor