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Tandem immunoprecipitation of phosphotyrosine-mass spectrometry (TIPY-MS) indicates C19ORF19 becomes tyrosine-phosphorylated and associated with activated epidermal growth factor receptor.

To identify phosphotyrosine (pY) sites in the epidermal growth factor receptor (EGFR) network, a tandem immunoprecipitation-mass spectrometry method (TIPY-MS) was applied wherein protease-digested EGFR immune complexes were extracted with anti-pY after Rush et al. ( Nat. Biotech. 2005, 23, 94 ) and analyzed by LC-MS/MS. New pY sites in the pathway were found, including SOS1 Y1065, SOS2 Y1275, CBL-B Y889, and in the EGFR regulatory protein Mig-6 Y458. The novel human C19orf19 gene product was found EGFR-associated and phosphorylated at 5 tyrosines in response to EGFR activation and, therefore, represents a new component of the EGFR signaling network.

Pubmed ID: 18271526


  • Tong J
  • Taylor P
  • Jovceva E
  • St-Germain JR
  • Jin LL
  • Nikolic A
  • Gu X
  • Li ZH
  • Trudel S
  • Moran MF


Journal of proteome research

Publication Data

March 10, 2008

Associated Grants


Mesh Terms

  • Amino Acid Sequence
  • Cell Line
  • Humans
  • Immunoprecipitation
  • Mass Spectrometry
  • Molecular Sequence Data
  • Open Reading Frames
  • Phosphorylation
  • Phosphotyrosine
  • Receptor, Epidermal Growth Factor