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Early involvement of synapsin III in neural progenitor cell development in the adult hippocampus.

Synapsin III is a synaptic vesicle-associated protein that is expressed in cells of the subgranular layer of the hippocampal dentate gyrus, a brain region known to sustain substantial levels of neurogenesis into adulthood. Here we tested the hypothesis that synapsin III plays a role in adult neurogenesis with synapsin III knockout and wild-type mice. Immunocytochemistry of the adult hippocampal dentate gyrus revealed that synapsin III colocalizes with markers of neural progenitor cell development (nestin, PSA-NCAM, NeuN, and Tuj1) but did not colocalize with markers of mitosis (Ki67 and PCNA). Because neurogenesis consists of a number of stages, the proliferation, survival, and differentiation of neural progenitor cells were systematically quantitated in the hippocampal dentate gyrus of adult synapsin III knockout and wild-type mice. We found a 30% decrease in proliferation and a 55% increase in survival of neural progenitor cells in synapsin III knockout mice. We also observed a 6% increase in the number of neural progenitor cells that differentiated into neurons. No difference in the volume of the dentate gyrus was observed between synapsin III knockout and wild-type mice. Collectively, our results demonstrate a novel role for synapsin III in regulating the proliferation of neural progenitor cells in the adult hippocampal dentate gyrus. These findings suggest a distinct function for this synaptic vesicle protein, in addition to its role in neurotransmission.

Pubmed ID: 18271024 RIS Download

Mesh terms: Age Factors | Animals | Biomarkers | Cell Differentiation | Cell Proliferation | Cell Survival | Dentate Gyrus | Female | Fluorescent Antibody Technique | Intermediate Filament Proteins | Male | Mice | Mice, Inbred C57BL | Mice, Knockout | Nerve Growth Factors | Nerve Tissue Proteins | Nestin | Neural Cell Adhesion Molecule L1 | Neuronal Plasticity | Neurons | Nuclear Proteins | Sialic Acids | Stem Cells | Synapsins | Time Factors | Tubulin

Antibody Registry (Reagent, Antibodies)

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