Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Nap1 links transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.

http://www.ncbi.nlm.nih.gov/pubmed/18227150

Chromatin remodeling is central to the regulation of transcription elongation. We demonstrate that the conserved Saccharomyces cerevisiae histone chaperone Nap1 associates with chromatin. We show that Nap1 regulates transcription of PHO5, and the increase in transcript level and the higher phosphatase activity plateau observed for Deltanap1 cells suggest that the net function of Nap1 is to facilitate nucleosome reassembly during transcription elongation. To further our understanding of histone chaperones in transcription elongation, we identified factors that regulate the function of Nap1 in this process. One factor investigated is an essential mRNA export and TREX complex component, Yra1. Nap1 interacts directly with Yra1 and genetically with other TREX complex components and the mRNA export factor Mex67. Additionally, we show that the recruitment of Nap1 to the coding region of actively transcribed genes is Yra1 dependent and that its recruitment to promoters is TREX complex independent. These observations suggest that Nap1 functions provide a new connection between transcription elongation, chromatin assembly, and messenger RNP complex biogenesis.

Pubmed ID: 18227150 RIS Download

Mesh terms: Acid Phosphatase | Cell Cycle Proteins | Chromatin Assembly and Disassembly | Gene Expression Regulation, Fungal | Multiprotein Complexes | Nuclear Proteins | Nucleocytoplasmic Transport Proteins | Nucleosome Assembly Protein 1 | Nucleosomes | Open Reading Frames | Protein Interaction Mapping | RNA Polymerase II | RNA Precursors | RNA Transport | RNA, Fungal | RNA, Messenger | RNA-Binding Proteins | Ribonucleoproteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Transcription, Genetic

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: R01 GM65385

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.