Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Regulatory divergence modifies limb length between mammals.

Genes & development | Jan 15, 2008

http://www.ncbi.nlm.nih.gov/pubmed/18198333

Natural selection acts on variation within populations, resulting in modified organ morphology, physiology, and ultimately the formation of new species. Although variation in orthologous proteins can contribute to these modifications, differences in DNA sequences regulating gene expression may be a primary source of variation. We replaced a limb-specific transcriptional enhancer of the mouse Prx1 locus with the orthologous sequence from a bat. Prx1 expression directed by the bat enhancer results in elevated transcript levels in developing forelimb bones and forelimbs that are significantly longer than controls because of endochondral bone formation alterations. Surprisingly, deletion of the mouse Prx1 limb enhancer results in normal forelimb length and Prx1 expression, revealing regulatory redundancy. These findings suggest that mutations accumulating in pre-existing noncoding regulatory sequences within a population are a source of variation for the evolution of morphological differences between species and that cis-regulatory redundancy may facilitate accumulation of such mutations.

Pubmed ID: 18198333 RIS Download

Mesh terms: Animals | Biological Evolution | Chiroptera | Enhancer Elements, Genetic | Evolution, Molecular | Forelimb | Gene Expression Regulation | Genes, Homeobox | Homeodomain Proteins | Mice | Mutation | Regulatory Sequences, Nucleic Acid | Transfection

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA 09299
  • Agency: NIDCR NIH HHS, Id: DE 16329
  • Agency: NICHD NIH HHS, Id: HD 16672
  • Agency: Intramural NIH HHS, Id:

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.