Preparing your results

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

A transcriptome database for astrocytes, neurons, and oligodendrocytes: a new resource for understanding brain development and function.

Understanding the cell-cell interactions that control CNS development and function has long been limited by the lack of methods to cleanly separate neural cell types. Here we describe methods for the prospective isolation and purification of astrocytes, neurons, and oligodendrocytes from developing and mature mouse forebrain. We used FACS (fluorescent-activated cell sorting) to isolate astrocytes from transgenic mice that express enhanced green fluorescent protein (EGFP) under the control of an S100beta promoter. Using Affymetrix GeneChip Arrays, we then created a transcriptome database of the expression levels of >20,000 genes by gene profiling these three main CNS neural cell types at various postnatal ages between postnatal day 1 (P1) and P30. This database provides a detailed global characterization and comparison of the genes expressed by acutely isolated astrocytes, neurons, and oligodendrocytes. We found that Aldh1L1 is a highly specific antigenic marker for astrocytes with a substantially broader pattern of astrocyte expression than the traditional astrocyte marker GFAP. Astrocytes were enriched in specific metabolic and lipid synthetic pathways, as well as the draper/Megf10 and Mertk/integrin alpha(v)beta5 phagocytic pathways suggesting that astrocytes are professional phagocytes. Our findings call into question the concept of a "glial" cell class as the gene profiles of astrocytes and oligodendrocytes are as dissimilar to each other as they are to neurons. This transcriptome database of acutely isolated purified astrocytes, neurons, and oligodendrocytes provides a resource to the neuroscience community by providing improved cell-type-specific markers and for better understanding of neural development, function, and disease.

Pubmed ID: 18171944


  • Cahoy JD
  • Emery B
  • Kaushal A
  • Foo LC
  • Zamanian JL
  • Christopherson KS
  • Xing Y
  • Lubischer JL
  • Krieg PA
  • Krupenko SA
  • Thompson WJ
  • Barres BA


The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

January 2, 2008

Associated Grants

  • Agency: NCI NIH HHS, Id: CA095030
  • Agency: NIDDK NIH HHS, Id: DK54388
  • Agency: NEI NIH HHS, Id: EY07033
  • Agency: NIGMS NIH HHS, Id: GM07365
  • Agency: NEI NIH HHS, Id: R01 EY10257
  • Agency: NINDS NIH HHS, Id: R01 NS045621

Mesh Terms

  • Animals
  • Astrocytes
  • Brain
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Green Fluorescent Proteins
  • Mice
  • Mice, Transgenic
  • Neurons
  • Oligodendroglia
  • Oligonucleotide Array Sequence Analysis
  • Transcription, Genetic