• Register
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.


Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.


L1 interaction with ankyrin regulates mediolateral topography in the retinocollicular projection.

Dynamic modulation of adhesion provided by anchorage of axonal receptors with the cytoskeleton contributes to attractant or repellent responses that guide axons to topographic targets in the brain. The neural cell adhesion molecule L1 engages the spectrin-actin cytoskeleton through reversible linkage of its cytoplasmic domain to ankyrin. To investigate a role for L1 association with the cytoskeleton in topographic guidance of retinal axons to the superior colliculus, a novel mouse strain was generated by genetic knock-in that expresses an L1 point mutation (Tyr1229His) abolishing ankyrin binding. Axon tracing revealed a striking mistargeting of mutant ganglion cell axons from the ventral retina, which express high levels of ephrinB receptors, to abnormally lateral sites in the contralateral superior colliculus, where they formed multiple ectopic arborizations. These axons were compromised in extending interstitial branches in the medial direction, a normal response to the high medial to low lateral SC gradient of ephrinB1. Furthermore, ventral but not dorsal L1(Y1229H) retinal cells were impaired for ephrinB1-stimulated adhesion through beta1 integrins in culture. The retinocollicular phenotype of the L1(Tyr1229His) mutant provides the first evidence that L1 regulates topographic mapping of retinal axons through adhesion mediated by linkage to the actin cytoskeleton and functional interaction with the ephrinB/EphB targeting system.

Pubmed ID: 18171935


  • Buhusi M
  • Schlatter MC
  • Demyanenko GP
  • Thresher R
  • Maness PF


The Journal of neuroscience : the official journal of the Society for Neuroscience

Publication Data

January 2, 2008

Associated Grants

  • Agency: PHS HHS, Id: R0149109

Mesh Terms

  • Amino Acids
  • Animals
  • Animals, Newborn
  • Ankyrins
  • Axons
  • Brain Mapping
  • Gene Expression Regulation, Developmental
  • Histidine
  • Immunoprecipitation
  • Mice
  • Mice, Transgenic
  • Mutation
  • Neural Cell Adhesion Molecule L1
  • Retina
  • Superior Colliculi
  • Tyrosine
  • Visual Pathways