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A role for the inositol kinase Ipk1 in ciliary beating and length maintenance.

Cilia project from cells as membranous extensions, with microtubule structural cores assembling from basal bodies by intraflagellar transport (IFT). Here, we report a ciliary role for the inositol 1,3,4,5,6-pentakisphosphate 2-kinase (Ipk1) that generates inositol hexakisphosphate. In zebrafish embryos, reducing Ipk1 levels inhibited ciliary beating in Kupffer's vesicle and decreased ciliary length in the spinal canal, pronephric ducts, and Kupffer's vesicle. Electron microscopy showed that ciliary axonemal structures were not grossly altered. However, coincident knockdown of Ipk1 and IFT88 or IFT57 had synergistic perturbations. With GFP-Ipk1 enriched in centrosomes and basal bodies, we propose that Ipk1 plays a previously uncharacterized role in ciliary function.

Pubmed ID: 18056639


  • Sarmah B
  • Winfrey VP
  • Olson GE
  • Appel B
  • Wente SR


Proceedings of the National Academy of Sciences of the United States of America

Publication Data

December 11, 2007

Associated Grants

  • Agency: NCRR NIH HHS, Id: 1S10RR15682
  • Agency: NCI NIH HHS, Id: CA68485
  • Agency: NIDDK NIH HHS, Id: DK20593
  • Agency: NIGMS NIH HHS, Id: GM51912
  • Agency: NINDS NIH HHS, Id: NS46668

Mesh Terms

  • Animals
  • Animals, Genetically Modified
  • Biological Transport
  • Body Patterning
  • Cell Line
  • Centrosome
  • Cilia
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Humans
  • Microtubules
  • Phosphotransferases (Alcohol Group Acceptor)
  • Protozoan Proteins
  • Zebrafish
  • Zebrafish Proteins