Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

NIX is required for programmed mitochondrial clearance during reticulocyte maturation.

http://www.ncbi.nlm.nih.gov/pubmed/18048346

The regulated clearance of mitochondria is a well recognized but poorly understood aspect of cellular homeostasis, and defects in this process have been linked to aging, degenerative diseases, and cancer. Mitochondria are recycled through an autophagy-related process, and reticulocytes, which completely eliminate their mitochondria during maturation, provide a physiological model to study this phenomenon. Here, we show that mitochondrial clearance in reticulocytes requires the BCL2-related protein NIX (BNIP3L). Mitochondrial clearance does not require BAX, BAK, BCL-X(L), BIM, or PUMA, indicating that NIX does not function through established proapoptotic pathways. Similarly, NIX is not required for the induction of autophagy during terminal erythroid differentiation. NIX is required for the selective elimination of mitochondria, however, because mitochondrial clearance, in the absence of NIX, is arrested at the stage of mitochondrial incorporation into autophagosomes and autophagosome maturation. These results yield insight into the mechanism of mitochondrial clearance in higher eukaryotes. Furthermore, they show a BAX- and BAK-independent role for a BCL2-related protein in development.

Pubmed ID: 18048346 RIS Download

Mesh terms: Animals | Apoptosis | Autophagy | Erythropoiesis | Humans | Membrane Proteins | Mice | Mice, Transgenic | Mitochondria | Proto-Oncogene Proteins | Proto-Oncogene Proteins c-bcl-2 | Reticulocytes | Tumor Suppressor Proteins | Ubiquitin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: R01 CA084214
  • Agency: NCI NIH HHS, Id: R01 CA084214-06A1
  • Agency: NCI NIH HHS, Id: R01 CA084214-07
  • Agency: NCI NIH HHS, Id: R01 CA084214-08
  • Agency: NCI NIH HHS, Id: R01 CA084214-09
  • Agency: NCI NIH HHS, Id: R01 CA084214-10
  • Agency: NIDDK NIH HHS, Id: R21 DK074519
  • Agency: NIDDK NIH HHS, Id: R21 DK074519-01
  • Agency: NIDDK NIH HHS, Id: R21 DK074519-02

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.