Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Nampt/PBEF/Visfatin regulates insulin secretion in beta cells as a systemic NAD biosynthetic enzyme.

Intracellular nicotinamide phosphoribosyltransferase (iNampt) is an essential enzyme in the NAD biosynthetic pathway. An extracellular form of this protein (eNampt) has been reported to act as a cytokine named PBEF or an insulin-mimetic hormone named visfatin, but its physiological relevance remains controversial. Here we show that eNampt does not exert insulin-mimetic effects in vitro or in vivo but rather exhibits robust NAD biosynthetic activity. Haplodeficiency and chemical inhibition of Nampt cause defects in NAD biosynthesis and glucose-stimulated insulin secretion in pancreatic islets in vivo and in vitro. These defects are corrected by administration of nicotinamide mononucleotide (NMN), a product of the Nampt reaction. A high concentration of NMN is present in mouse plasma, and plasma eNampt and NMN levels are reduced in Nampt heterozygous females. Our results demonstrate that Nampt-mediated systemic NAD biosynthesis is critical for beta cell function, suggesting a vital framework for the regulation of glucose homeostasis.

Pubmed ID: 17983582


  • Revollo JR
  • K├Ârner A
  • Mills KF
  • Satoh A
  • Wang T
  • Garten A
  • Dasgupta B
  • Sasaki Y
  • Wolberger C
  • Townsend RR
  • Milbrandt J
  • Kiess W
  • Imai S


Cell metabolism

Publication Data

November 6, 2007

Associated Grants

  • Agency: NIA NIH HHS, Id: AG024150
  • Agency: NCRR NIH HHS, Id: C06RR015502
  • Agency: NIDDK NIH HHS, Id: DK56341
  • Agency: NINDS NIH HHS, Id: NS36358
  • Agency: NIDDK NIH HHS, Id: P30 DK056341
  • Agency: NIDDK NIH HHS, Id: P30 DK056341-06
  • Agency: NIDDK NIH HHS, Id: P30 DK056341-07
  • Agency: NIDDK NIH HHS, Id: P30 DK52574
  • Agency: NCRR NIH HHS, Id: P41RR00945
  • Agency: NIA NIH HHS, Id: R01 AG024150
  • Agency: NIA NIH HHS, Id: R01 AG024150-04

Mesh Terms

  • Adipose Tissue, Brown
  • Animals
  • Cell Differentiation
  • Cell Line
  • Female
  • Glucose Intolerance
  • Immunoprecipitation
  • Insulin
  • Insulin-Secreting Cells
  • Islets of Langerhans
  • Kidney
  • Liver
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • Myocardium
  • NAD
  • Nicotinamide Mononucleotide
  • Nicotinamide Phosphoribosyltransferase
  • Signal Transduction