Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

The structure of the gamma-tubulin small complex: implications of its architecture and flexibility for microtubule nucleation.

http://www.ncbi.nlm.nih.gov/pubmed/17978090

The gamma-tubulin small complex (gamma-TuSC) is an evolutionarily conserved heterotetramer essential for microtubule nucleation. We have determined the structure of the Saccharomyces cerevisiae gamma-TuSC at 25-A resolution by electron microscopy. gamma-TuSC is Y-shaped, with an elongated body connected to two arms. Gold labeling showed that the two gamma-tubulins are located in lobes at the ends of the arms, and the relative orientations of the other gamma-TuSC components were determined by in vivo FRET. The structures of different subpopulations of gamma-TuSC indicate flexibility in the connection between a mobile arm and the rest of the complex, resulting in variation of the relative positions and orientations of the gamma-tubulins. In all of the structures, the gamma-tubulins are distinctly separated, a configuration incompatible with the microtubule lattice. The separation of the gamma-tubulins in isolated gamma-TuSC likely plays a role in suppressing its intrinsic microtubule-nucleating activity, which is relatively weak until the gamma-TuSC is incorporated into higher order complexes or localized to microtubule-organizing centers. We propose that further movement of the mobile arm is required to bring the gamma-tubulins together in microtubule-like interactions, and provide a template for microtubule growth.

Pubmed ID: 17978090 RIS Download

Mesh terms: Fluorescence Resonance Energy Transfer | Microscopy, Electron | Microtubules | Models, Molecular | Nucleotides | Pliability | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Tubulin

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIGMS NIH HHS, Id: F32 GM-078790
  • Agency: NCRR NIH HHS, Id: P41 RR-011823
  • Agency: NCRR NIH HHS, Id: P41 RR011823
  • Agency: NIGMS NIH HHS, Id: R01 GM-040506
  • Agency: NIGMS NIH HHS, Id: R01 GM-31627
  • Agency: Howard Hughes Medical Institute, Id:

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.