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The PIAS-like protein Zimp10 is essential for embryonic viability and proper vascular development.

http://www.ncbi.nlm.nih.gov/pubmed/17967885

Members of the PIAS (for protein inhibitor of activated STAT) family play critical roles in modulating the activity of a variety of transcriptional regulators. Zimp10, a novel PIAS-like protein, is a transcriptional coregulator and may be involved in the modification of chromatin through interactions with the SWI/SNF chromatin-remodeling complexes. Here, we investigate the biological role of Zimp10 in zimp10-deficient mice. Homozygosity for the Zimp10-targeted allele resulted in developmental arrest at approximately embryonic day 10.5. Analysis of knockout embryos revealed severe defects in the reorganization of the yolk sac vascular plexus. No significant abnormality in hematopoietic potential was observed in zimp10 null mice. Microarray and quantified reverse transcription-PCR analyses showed that the expression of the Fos family member Fra-1, which is involved in extraembryonic vascular development, was reduced in yolk sac tissues of zimp10 null embryos. Using fra-1 promoter/reporter constructs, we further demonstrate the regulatory role of Zimp10 on the transcription of Fra-1. This study provides evidence to demonstrate a crucial role for Zimp10 in vasculogenesis.

Pubmed ID: 17967885 RIS Download

Mesh terms: Animals | Cells, Cultured | Embryo Loss | Embryo, Mammalian | Female | Gene Expression Regulation, Developmental | Hematopoiesis | Homozygote | Humans | Mice | Mice, Knockout | Oligonucleotide Array Sequence Analysis | Pregnancy | Proto-Oncogene Proteins c-fos | Transcription Factors | Yolk Sac

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Associated grants

  • Agency: NCI NIH HHS, Id: 5T32 CA09302
  • Agency: NCI NIH HHS, Id: CA070297
  • Agency: NCI NIH HHS, Id: CA087767
  • Agency: NIDDK NIH HHS, Id: DK061002
  • Agency: NCI NIH HHS, Id: R01 CA070297
  • Agency: NCI NIH HHS, Id: R01 CA070297-09
  • Agency: NCI NIH HHS, Id: R01 CA070297-10
  • Agency: NCI NIH HHS, Id: R01 CA070297-11A2
  • Agency: NCI NIH HHS, Id: R01 CA070297-12
  • Agency: NCI NIH HHS, Id: R01 CA087767
  • Agency: NIDDK NIH HHS, Id: R01 DK061002
  • Agency: NIDDK NIH HHS, Id: R56 DK061002

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