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Two-photon photostimulation and imaging of neural circuits.

Nature methods | Nov 31, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17965719

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

Pubmed ID: 17965719 RIS Download

Mesh terms: Acetates | Action Potentials | Animals | Brain Mapping | Calcium Signaling | Cytophotometry | Electrophysiology | Excitatory Postsynaptic Potentials | Glutamates | Glutamine | Indoles | Mice | Mice, Inbred C57BL | Mice, Transgenic | Microscopy, Fluorescence, Multiphoton | Neocortex | Nerve Net | Neurons | Reproducibility of Results | Tetrodotoxin

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