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Two-photon photostimulation and imaging of neural circuits.

We introduce an optical method to stimulate individual neurons in brain slices in any arbitrary spatiotemporal pattern, using two-photon uncaging of MNI-glutamate with beam multiplexing. This method has single-cell and three-dimensional precision. By sequentially stimulating up to a thousand potential presynaptic neurons, we generated detailed functional maps of inputs to a cell. We combined this approach with two-photon calcium imaging in an all-optical method to image and manipulate circuit activity.

Pubmed ID: 17965719


  • Nikolenko V
  • Poskanzer KE
  • Yuste R


Nature methods

Publication Data

November 31, 2007

Associated Grants


Mesh Terms

  • Acetates
  • Action Potentials
  • Animals
  • Brain Mapping
  • Calcium Signaling
  • Cytophotometry
  • Electrophysiology
  • Excitatory Postsynaptic Potentials
  • Glutamates
  • Glutamine
  • Indoles
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Microscopy, Fluorescence, Multiphoton
  • Neocortex
  • Nerve Net
  • Neurons
  • Reproducibility of Results
  • Tetrodotoxin