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Mind bomb-2 is an E3 ligase that ubiquitinates the N-methyl-D-aspartate receptor NR2B subunit in a phosphorylation-dependent manner.

http://www.ncbi.nlm.nih.gov/pubmed/17962190

The N-methyl-D-aspartate receptor (NMDAR) plays a critical role in synaptic plasticity. Post-translational modifications of NMDARs, such as phosphorylation, alter both the activity and trafficking properties of NMDARs. Ubiquitination is increasingly being recognized as another post-translational modification that can alter synaptic protein composition and function. We identified Mind bomb-2 as an E3 ubiquitin ligase that interacts with and ubiquitinates the NR2B subunit of the NMDAR in mammalian cells. The protein-protein interaction and the ubiquitination of the NR2B subunit were found to be enhanced in a Fyn phosphorylation-dependent manner. Immunocytochemical studies reveal that Mind bomb-2 is localized to postsynaptic sites and colocalizes with the NMDAR in apical dendrites of hippocampal neurons. Furthermore, we show that NMDAR activity is down-regulated by Mind bomb-2. These results identify a specific E3 ubiquitin ligase as a novel interactant with the NR2B subunit and suggest a possible mechanism for the regulation of NMDAR function involving both phosphorylation and ubiquitination.

Pubmed ID: 17962190 RIS Download

Mesh terms: Animals | Cell Line | Cells, Cultured | Hippocampus | Humans | Immunoprecipitation | In Situ Hybridization | Models, Biological | Neurons | Phosphorylation | Protein Binding | Protein Processing, Post-Translational | Proto-Oncogene Proteins c-fyn | Rats | Receptors, N-Methyl-D-Aspartate | Two-Hybrid System Techniques | Ubiquitin | Ubiquitin-Protein Ligases | Ubiquitination

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Associated grants

  • Agency: NIAAA NIH HHS, Id: R01 AA 013438-01A1
  • Agency: NIAAA NIH HHS, Id: R01 AA013438
  • Agency: NIAAA NIH HHS, Id: R01 AA013438-01A1

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