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Dynamic clustered distribution of hemagglutinin resolved at 40 nm in living cell membranes discriminates between raft theories.

Organization in biological membranes spans many orders of magnitude in length scale, but limited resolution in far-field light microscopy has impeded distinction between numerous biomembrane models. One canonical example of a heterogeneously distributed membrane protein is hemagglutinin (HA) from influenza virus, which is associated with controversial cholesterol-rich lipid rafts. Using fluorescence photoactivation localization microscopy, we are able to image distributions of tens of thousands of HA molecules with subdiffraction resolution ( approximately 40 nm) in live and fixed fibroblasts. HA molecules form irregular clusters on length scales from approximately 40 nm up to many micrometers, consistent with results from electron microscopy. In live cells, the dynamics of HA molecules within clusters is observed and quantified to determine an effective diffusion coefficient. The results are interpreted in terms of several established models of biological membranes.

Pubmed ID: 17959773

Authors

  • Hess ST
  • Gould TJ
  • Gudheti MV
  • Maas SA
  • Mills KD
  • Zimmerberg J

Journal

Proceedings of the National Academy of Sciences of the United States of America

Publication Data

October 30, 2007

Associated Grants

  • Agency: NIAID NIH HHS, Id: K25AI65459
  • Agency: Intramural NIH HHS, Id:

Mesh Terms

  • Cell Line
  • Cell Survival
  • Hemagglutinins
  • Membrane Microdomains
  • Nanostructures