MiR-150 controls B cell differentiation by targeting the transcription factor c-Myb.
MiR-150 is a microRNA (miRNA) specifically expressed in mature lymphocytes, but not their progenitors. A top predicted target of miR-150 is c-Myb, a transcription factor controlling multiple steps of lymphocyte development. Combining loss- and gain-of-function gene targeting approaches for miR-150 with conditional and partial ablation of c-Myb, we show that miR-150 indeed controls c-Myb expression in vivo in a dose-dependent manner over a narrow range of miRNA and c-Myb concentrations and that this dramatically affects lymphocyte development and response. Our results identify a key transcription factor as a critical target of a stage-specifically expressed miRNA in lymphocytes and suggest that this and perhaps other miRNAs have evolved to control the expression of just a few critical target proteins in particular cellular contexts.
Pubmed ID: 17923094 RIS Download
3' Untranslated Regions | Animals | B-Lymphocytes | Cell Death | Cell Differentiation | Cells, Cultured | Gene Expression Regulation | Gene Targeting | Genes, Reporter | Humans | Immune System | Mice | Mice, Knockout | MicroRNAs | Proto-Oncogene Proteins c-myb | T-Lymphocytes