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Comprehensive analysis of diverse ribonucleoprotein complexes.

Nature methods | Nov 31, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17922018

The study of the dynamic interactome of cellular ribonucleoprotein (RNP) particles has been hampered by severe methodological limitations. In particular, the affinity purification of intact RNP complexes from cell lysates suffers from RNA degradation, loss of interacting macromolecules and poor overall yields. Here we describe a rapid affinity-purification method for efficient isolation of the subcomplexes that dynamically organize different RNP biogenesis pathways in Saccharomyces cerevisiae. Our method overcomes many of the previous limitations to produce large RNP interactomes with almost no contamination.

Pubmed ID: 17922018 RIS Download

Mesh terms: DNA-Binding Proteins | Electrophoresis, Polyacrylamide Gel | Green Fluorescent Proteins | Immunosorbent Techniques | Mass Spectrometry | Nuclear Proteins | Nucleocytoplasmic Transport Proteins | Oligonucleotide Array Sequence Analysis | Porins | RNA Cap-Binding Proteins | RNA, Fungal | RNA, Messenger | RNA, Ribosomal | RNA-Binding Proteins | Ribonucleoproteins | Ribosomal Proteins | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Staphylococcal Protein A

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM067228
  • Agency: NIGMS NIH HHS, Id: GM076547
  • Agency: NCRR NIH HHS, Id: RR00862
  • Agency: NCRR NIH HHS, Id: RR022220
  • Agency: NCRR NIH HHS, Id: U54 RR022220

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