• Register
X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

X

Leaving Community

Are you sure you want to leave this community? Leaving the community will revoke any permissions you have been granted in this community.

No
Yes

Disruption of the basal body compromises proteasomal function and perturbs intracellular Wnt response.

Primary cilia and basal bodies are evolutionarily conserved organelles that mediate communication between the intracellular and extracellular environments. Here we show that bbs1, bbs4 and mkks (also known as bbs6), which encode basal body proteins, are required for convergence and extension in zebrafish and interact with wnt11 and wnt5b. Suppression of bbs1, bbs4 and mkks transcripts results in stabilization of beta-catenin with concomitant upregulation of T-cell factor (TCF)-dependent transcription in both zebrafish embryos and mammalian ciliated cells, a defect phenocopied by the silencing of the axonemal kinesin subunit KIF3A but not by chemical disruption of the cytoplasmic microtubule network. These observations are attributable partly to defective degradation by the proteasome; suppression of BBS4 leads to perturbed proteasomal targeting and concomitant accumulation of cytoplasmic beta-catenin. Cumulatively, our data indicate that the basal body is an important regulator of Wnt signal interpretation through selective proteolysis and suggest that defects in this system may contribute to phenotypes pathognomonic of human ciliopathies.

Pubmed ID: 17906624

Authors

  • Gerdes JM
  • Liu Y
  • Zaghloul NA
  • Leitch CC
  • Lawson SS
  • Kato M
  • Beachy PA
  • Beales PL
  • DeMartino GN
  • Fisher S
  • Badano JL
  • Katsanis N

Journal

Nature genetics

Publication Data

November 30, 2007

Associated Grants

  • Agency: Medical Research Council, Id: G0801843
  • Agency: Wellcome Trust, Id:

Mesh Terms

  • Animals
  • Cells, Cultured
  • Ciliary Body
  • Cytoplasm
  • Cytoskeleton
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental
  • Group II Chaperonins
  • Humans
  • In Situ Hybridization
  • Kidney
  • Kinesin
  • Luciferases
  • Microinjections
  • Microtubules
  • Phenotype
  • Phosphorylation
  • Proteasome Endopeptidase Complex
  • Proteasome Inhibitors
  • TCF Transcription Factors
  • Transcription, Genetic
  • Wnt Proteins
  • Zebrafish
  • Zebrafish Proteins
  • beta Catenin