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Differences in the single-stranded DNA binding activities of MCM2-7 and MCM467: MCM2 and MCM5 define a slow ATP-dependent step.

http://www.ncbi.nlm.nih.gov/pubmed/17895243

The MCM2-7 complex, a hexamer containing six distinct and essential subunits, is postulated to be the eukaryotic replicative DNA helicase. Although all six subunits function at the replication fork, only a specific subcomplex consisting of the MCM4, 6, and 7 subunits (MCM467) and not the MCM2-7 complex exhibits DNA helicase activity in vitro. To understand why MCM2-7 lacks helicase activity and to address the possible function of the MCM2, 3, and 5 subunits, we have compared the biochemical properties of the Saccharomyces cerevisiae MCM2-7 and MCM467 complexes. We demonstrate that both complexes are toroidal and possess a similar ATP-dependent single-stranded DNA (ssDNA) binding activity, indicating that the lack of helicase activity by MCM2-7 is not due to ineffective ssDNA binding. We identify two important differences between them. MCM467 binds dsDNA better than MCM2-7. In addition, we find that the rate of MCM2-7/ssDNA association is slow compared with MCM467; the association rate can be dramatically increased either by preincubation with ATP or by inclusion of mutations that ablate the MCM2/5 active site. We propose that the DNA binding differences between MCM2-7 and MCM467 correspond to a conformational change at the MCM2/5 active site with putative regulatory significance.

Pubmed ID: 17895243 RIS Download

Mesh terms: Adenosine Triphosphate | Binding Sites | Binding, Competitive | Cell Cycle Proteins | Chromosomal Proteins, Non-Histone | DNA Helicases | DNA, Single-Stranded | DNA-Binding Proteins | Dose-Response Relationship, Drug | Fungal Proteins | Kinetics | Minichromosome Maintenance Complex Component 4 | Minichromosome Maintenance Complex Component 6 | Models, Biological | Molecular Conformation | Nucleotides | Protein Binding | Protein Structure, Tertiary | Saccharomyces cerevisiae | Saccharomyces cerevisiae Proteins | Time Factors

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