Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Human ESCRT and ALIX proteins interact with proteins of the midbody and function in cytokinesis.

The EMBO journal | Oct 3, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17853893

TSG101 and ALIX both function in HIV budding and in vesicle formation at the multivesicular body (MVB), where they interact with other Endosomal Sorting Complex Required for Transport (ESCRT) pathway factors required for release of viruses and vesicles. Proteomic analyses revealed that ALIX and TSG101/ESCRT-I also bind a series of proteins involved in cytokinesis, including CEP55, CD2AP, ROCK1, and IQGAP1. ALIX and TSG101 concentrate at centrosomes and are then recruited to the midbodies of dividing cells through direct interactions between the central CEP55 'hinge' region and GPP-based motifs within TSG101 and ALIX. ESCRT-III and VPS4 proteins are also recruited, indicating that much of the ESCRT pathway localizes to the midbody. Depletion of ALIX and TSG101/ESCRT-I inhibits the abscission step of HeLa cell cytokinesis, as does VPS4 overexpression, confirming a requirement for these proteins in cell division. Furthermore, ALIX point mutants that block CEP55 and CHMP4/ESCRT-III binding also inhibit abscission, indicating that both interactions are essential. These experiments suggest that the ESCRT pathway may be recruited to facilitate analogous membrane fission events during HIV budding, MVB vesicle formation, and the abscission stage of cytokinesis.

Pubmed ID: 17853893 RIS Download

Mesh terms: Amino Acid Motifs | Animals | COS Cells | Calcium-Binding Proteins | Carrier Proteins | Cell Cycle Proteins | Centrosome | Cercopithecus aethiops | Cytokinesis | DNA-Binding Proteins | Endosomal Sorting Complexes Required for Transport | Endosomes | HIV | HeLa Cells | Humans | Multiprotein Complexes | Protein Binding | Protein Structure, Tertiary | Transcription Factors | Virus Assembly | Virus Internalization

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NIAID NIH HHS, Id: AI051174
  • Agency: NIAID NIH HHS, Id: AI45405
  • Agency: NIAID NIH HHS, Id: R01 AI051174

BioGRID (Data, Interactions)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.