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Disruption of BCATm in mice leads to increased energy expenditure associated with the activation of a futile protein turnover cycle.

Leucine is recognized as a nutrient signal; however, the long-term in vivo consequences of leucine signaling and the role of branched-chain amino acid (BCAA) metabolism in this signaling remain unclear. To investigate these questions, we disrupted the BCATm gene, which encodes the enzyme catalyzing the first step in peripheral BCAA metabolism. BCATm(-/-) mice exhibited elevated plasma BCAAs and decreased adiposity and body weight, despite eating more food, along with increased energy expenditure, remarkable improvements in glucose and insulin tolerance, and protection from diet-induced obesity. The increased energy expenditure did not seem to be due to altered locomotor activity, uncoupling proteins, sympathetic activity, or thyroid hormones but was strongly associated with food consumption and an active futile cycle of increased protein degradation and synthesis. These observations suggest that elevated BCAAs and/or loss of BCAA catabolism in peripheral tissues play an important role in regulating insulin sensitivity and energy expenditure.

Pubmed ID: 17767905

Authors

  • She P
  • Reid TM
  • Bronson SK
  • Vary TC
  • Hajnal A
  • Lynch CJ
  • Hutson SM

Journal

Cell metabolism

Publication Data

September 4, 2007

Associated Grants

  • Agency: NIAAA NIH HHS, Id: AA12814
  • Agency: NIDDK NIH HHS, Id: DK053843
  • Agency: NIDDK NIH HHS, Id: DK062880
  • Agency: NIDDK NIH HHS, Id: DK34738
  • Agency: NIGMS NIH HHS, Id: GM39722
  • Agency: NINDS NIH HHS, Id: NS038641
  • Agency: NIDDK NIH HHS, Id: R01 DK053843
  • Agency: NIDDK NIH HHS, Id: R01 DK053843-09
  • Agency: NIDDK NIH HHS, Id: R01 DK062880
  • Agency: NIDDK NIH HHS, Id: R01 DK062880-06
  • Agency: NIDDK NIH HHS, Id: R01 DK062880-07

Mesh Terms

  • Adipose Tissue
  • Animals
  • Body Weight
  • Diet
  • Eating
  • Energy Metabolism
  • Female
  • Gene Targeting
  • Glucose Tolerance Test
  • Humans
  • Insulin
  • Leucine
  • Male
  • Mice
  • Mice, Knockout
  • Obesity
  • Organ Size
  • Oxygen Consumption
  • Protein Kinases
  • Proteins
  • Sirolimus
  • Substrate Cycling
  • TOR Serine-Threonine Kinases
  • Thermogenesis
  • Transaminases