Preparing your results

Our searching services are busy right now. Your search will reload in five seconds.

X
Forgot Password

If you have forgotten your password you can enter your email here and get a temporary password sent to your email.

Polysialic acid-directed migration and differentiation of neural precursors are essential for mouse brain development.

http://www.ncbi.nlm.nih.gov/pubmed/17682066

Polysialic acid, which is synthesized by two polysialyltransferases, ST8SiaII and ST8SiaIV, plays an essential role in brain development by modifying the neural cell adhesion molecule (NCAM). It is currently unclear how polysialic acid functions in different processes of neural development. Here we generated mice doubly mutant in both ST8SiaII and ST8SiaIV to determine the effects of loss of polysialic acid on brain development. In contrast to NCAM-deficient, ST8SiaII-deficient, or ST8SiaIV-deficient single mutant mice, ST8SiaII and ST8SiaIV double mutants displayed severe defects in anatomical organization of the forebrain associated with apoptotic cell death. Loss of polysialic acid affected both tangential and radial migration of neural precursors during cortical development, resulting in aberrant positioning of neuronal and glial cells. Glial cell differentiation was aberrantly increased in vivo and in vitro in the absence of polysialic acid. Consistent with these findings, polysialic acid-deficient mice exhibited increased expression of the glial cell marker glial fibrillary acidic protein and a decrease in expression of Pax6, a transcription factor regulating neural cell migration. These results indicate that polysialic acid regulates cell migration and differentiation of neural precursors crucial for brain development.

Pubmed ID: 17682066 RIS Download

Mesh terms: Animals | Apoptosis | Cell Differentiation | Cell Movement | Cerebral Cortex | Eye Proteins | Glial Fibrillary Acidic Protein | Homeodomain Proteins | Isoenzymes | Luminescent Proteins | Mice | Mice, Knockout | Neuroglia | Neurons | Paired Box Transcription Factors | Repressor Proteins | Sialic Acids | Sialyltransferases | Stem Cells

Research resources used in this publication

None found

Research tools detected in this publication

None found

Data used in this publication

None found

Associated grants

  • Agency: NCI NIH HHS, Id: CA33895
  • Agency: NICHD NIH HHS, Id: HD25938
  • Agency: NHLBI NIH HHS, Id: HL57345
  • Agency: NINDS NIH HHS, Id: NS47351

Mouse Genome Informatics (Data, Gene Annotation)

Publication data is provided by the National Library of Medicine ® and PubMed ®. Data is retrieved from PubMed ® on a weekly schedule. For terms and conditions see the National Library of Medicine Terms and Conditions.