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Seizures and reduced life span in mice lacking the potassium channel subunit Kv1.2, but hypoexcitability and enlarged Kv1 currents in auditory neurons.

http://www.ncbi.nlm.nih.gov/pubmed/17634333

Genes Kcna1 and Kcna2 code for the voltage-dependent potassium channel subunits Kv1.1 and Kv1.2, which are coexpressed in large axons and commonly present within the same tetramers. Both contribute to the low-voltage-activated potassium current I Kv1, which powerfully limits excitability and facilitates temporally precise transmission of information, e.g., in auditory neurons of the medial nucleus of the trapezoid body (MNTB). Kcna1-null mice lacking Kv1.1 exhibited seizure susceptibility and hyperexcitability in axons and MNTB neurons, which also had reduced I Kv1. To explore whether a lack of Kv1.2 would cause a similar phenotype, we created and characterized Kcna2-null mice (-/-). The -/- mice exhibited increased seizure susceptibility compared with their +/+ and +/- littermates, as early as P14. The mRNA for Kv1.1 and Kv1.2 increased strongly in +/+ brain stems between P7 and P14, suggesting the increasing importance of these subunits for limiting excitability. Surprisingly, MNTB neurons in brain stem slices from -/- and +/- mice were hypoexcitable despite their Kcna2 deficit, and voltage-clamped -/- MNTB neurons had enlarged I Kv1. This contrasts strikingly with the Kcna1-null MNTB phenotype. Toxin block experiments on MNTB neurons suggested Kv1.2 was present in every +/+ Kv1 channel, about 60% of +/- Kv1 channels, and no -/- Kv1 channels. Kv1 channels lacking Kv1.2 activated at abnormally negative potentials, which may explain why MNTB neurons with larger proportions of such channels had larger I Kv1. If channel voltage dependence is determined by how many Kv1.2 subunits each contains, neurons might be able to fine-tune their excitability by adjusting the Kv1.1:Kv1.2 balance rather than altering Kv1 channel density.

Pubmed ID: 17634333 RIS Download

Mesh terms: Aging | Animals | Brain Stem | Genetic Vectors | Genome | Genotype | Kv1.2 Potassium Channel | Life Expectancy | Mice | Mice, Knockout | Neurons | Open Reading Frames | Restriction Mapping | Seizures | Shaker Superfamily of Potassium Channels

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Associated grants

  • Agency: NIDCD NIH HHS, Id: DC-003805
  • Agency: NIDCD NIH HHS, Id: DC-02739
  • Agency: NIDCD NIH HHS, Id: P30-DC-04661
  • Agency: NIDCD NIH HHS, Id: T32-DC-05361
  • Agency: NIGMS NIH HHS, Id: T32-GM-07108

Mouse Genome Informatics (Data, Gene Annotation)

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