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Array tomography: a new tool for imaging the molecular architecture and ultrastructure of neural circuits.

Neuron | Jul 5, 2007

Many biological functions depend critically upon fine details of tissue molecular architecture that have resisted exploration by existing imaging techniques. This is particularly true for nervous system tissues, where information processing function depends on intricate circuit and synaptic architectures. Here, we describe a new imaging method, called array tomography, which combines and extends superlative features of modern optical fluorescence and electron microscopy methods. Based on methods for constructing and repeatedly staining and imaging ordered arrays of ultrathin (50-200 nm), resin-embedded serial sections on glass microscope slides, array tomography allows for quantitative, high-resolution, large-field volumetric imaging of large numbers of antigens, fluorescent proteins, and ultrastructure in individual tissue specimens. Compared to confocal microscopy, array tomography offers the advantage of better spatial resolution, in particular along the z axis, as well as depth-independent immunofluorescent staining. The application of array tomography can reveal important but previously unseen features of brain molecular architecture.

Pubmed ID: 17610815 RIS Download

Mesh terms: Animals | Diagnostic Imaging | Fluorescent Antibody Technique | Fluorescent Dyes | Green Fluorescent Proteins | Image Processing, Computer-Assisted | Immunohistochemistry | Male | Microscopy, Confocal | Microscopy, Electron, Scanning | Nerve Net | Neurology | Photobleaching | Rats | Rats, Sprague-Dawley | Synapsins | Tissue Embedding

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Associated grants

  • Agency: NINDS NIH HHS, Id: R01 NS054252-02

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Amira 3D analysis

Software tool for visualizing, manipulating, and understanding data from tomography, microscopy, MRI and other imaging processes.Used to import and export options, to processes 3D image filtering and DTI based fiber tracking to visualization, volume and surface rendering, author tools for virtual reality navigation, video generation, and more.

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Advanced 3D Visualization and Volume Modeling

Software tool for visualizing, manipulating, and understanding data from tomography, microscopy, MRI and other imaging processes.Used to import and export options, to processes 3D image filtering and DTI based fiber tracking to visualization, volume and surface rendering, author tools for virtual reality navigation, video generation, and more.

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