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Bright monomeric red fluorescent protein with an extended fluorescence lifetime.

Nature methods | Jul 29, 2007

http://www.ncbi.nlm.nih.gov/pubmed/17572680

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

Pubmed ID: 17572680 RIS Download

Mesh terms: Fluorescence | Fluorescence Resonance Energy Transfer | HeLa Cells | Humans | Luminescent Agents | Luminescent Proteins | Proteins

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Associated grants

  • Agency: NIGMS NIH HHS, Id: GM070358

Mouse Genome Informatics (Data, Gene Annotation)

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