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Bright monomeric red fluorescent protein with an extended fluorescence lifetime.

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.

Pubmed ID: 17572680

Authors

  • Merzlyak EM
  • Goedhart J
  • Shcherbo D
  • Bulina ME
  • Shcheglov AS
  • Fradkov AF
  • Gaintzeva A
  • Lukyanov KA
  • Lukyanov S
  • Gadella TW
  • Chudakov DM

Journal

Nature methods

Publication Data

July 29, 2007

Associated Grants

  • Agency: NIGMS NIH HHS, Id: GM070358

Mesh Terms

  • Fluorescence
  • Fluorescence Resonance Energy Transfer
  • HeLa Cells
  • Humans
  • Luminescent Agents
  • Luminescent Proteins
  • Proteins